Figure 7.

Effect of RF-Id on carrageenan-induced oedema and on zymosan-induced cell migration and LTB₄ and PGE₂ levels in vivo. (A) Carrageenan-induced oedema. Mice were divided into four groups (n = 6) and received i.p. administration of RF-Id (0.1, 1 or 10 mg·kg⁻¹ 30 min before subplantar injection of 50 μL of carrageenan (1%, w v⁻¹). Paw volume was measured using a hydroplethismometer, specially modified for small volumes, immediately before the subplantar injection (basal value) and 2, 4, 6, 24, 48 and 72 h thereafter. Data are means ± SEM., n = 6. *P <0.05; ***P <0.001; 1 mg·kg⁻¹: ††P <0.01; †††P <0.001; ++P <0.01; +++P <0.001. (B–D) Cell migration in air pouches of mice. Mice (n = 5–6 per group) received i.p. administration of RF-Id (0.1 and 1 mg·kg⁻¹), indomethacin (2.5 mg·kg⁻¹) or vehicle (veh, DMSO). After 30 min, air pouches were developed by subcutaneous injection of 2.5 mL sterile air into the backs of the mice. Zymosan 1% (w v⁻¹) was used as an inflammatory agent to induce cell migration. After 4 h of zymosan injection, mice were killed by CO₂ exposure and exudate in the pouch was collected and the total leukocyte count was evaluated by optical microscopy of the cell suspension diluted with Turk's solution (B). The supernatants of the exudate were analysed for LTB₄ (C) or PGE₂ (D) by elisa. Data are means + SEM., n = 5–6. *P <0.05, anova + Bonferroni (B) and Student's t-test (C).