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. 2014 Apr 11;171(9):2399–2412. doi: 10.1111/bph.12592

Figure 8.

Figure 8

Effects of RF-Id on various lipoxygenases and COX-2 activity. (A) Neutrophil homogenates were incubated with RF-Id for 15 min at 4°C. Left panel: without DTT; right panel with 1 mM DTT, added 5 min before stimulation. After addition of 1 mM ATP, samples were warmed up for 30 s at 37°C and stimulated with 2 mM CaCl2 and 20 μM AA for 10 min at 37°C. 5-LOX products, 12-H(P)ETE and 15-H(P)ETE were extracted and analysed by HPLC. Data are expressed as percentage of vehicle control (0.1% DMSO); means ± SEM; n = 3–4. (B) Effect of RF-Id on the formation of the COX-2-derived 6-keto PGF1α. IL-1β-stimulated A549 cells were pre-incubated with RF-Id for 15 min at 37°C and stimulated with 3 μM AA for 15 min at 37°C. Then 6-keto PGF1α was analysed by elisa. Data are expressed as percentage of vehicle control (0.1% DMSO); means ± SEM; n = 3. *P <0.05, ***P <0.001 versus vehicle control.