Table 1.

ALP activity and evaluation of undifferentiation markers in Fld-ES cells

Marker	FGF2 added		No FGF2 added
Before passage		Passage 1	Passage 10	Passage 20
ALP	+		+	+	+
SSEA-1	–		–	–	–
SSEA-3	–		–	–	–
SSEA-4	+		+	+	+
TRA-1-60	+		+	+	+
TRA-1-81	+		+	+	+
Formation of EB	+		+	+	+

^* P, passage numbers.