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. Author manuscript; available in PMC: 2015 Feb 6.
Published in final edited form as: J Mol Biol. 2013 Nov 8;426(3):645–655. doi: 10.1016/j.jmb.2013.11.002

Figure 3. Comparative enzymatic activities of split and intact PE3.

Figure 3

ADPRT activity was measured by incorporation of 6-carboxyfluorescein-17-NAD+ into yeast eEF2 as described in Materials and Methods. Concentrations for split PE3 refer to the PEα:PEβ complex (harboring a heterodimeric coiled coil) or PE-A+PE-B (coil-free). The coil-free fragments do not heterodimerize as judged by pull-down experiments (Figure S2, Supplementary Data). Relative fluorescence incorporation was normalized against the fluorescence of a mock sample containing fluorescein-NAD+ alone. Symbols represent means ± SEM from duplicate measurements. Lines are best fits to the Hill equation.