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. Author manuscript; available in PMC: 2015 Feb 6.
Published in final edited form as: J Mol Biol. 2013 Nov 8;426(3):645–655. doi: 10.1016/j.jmb.2013.11.002

Figure 6. Co-transfection of split PE3 causes cell death.

Figure 6

HEK293 cells were transiently transfected with expression plasmids encoding the indicated constructs and incubated for 48 or 72 h. A constant amount of total DNA (500 ng per 105 cells) was used in each treatment. A, Cytotoxicity relative to intact PE3 was measured by XTT reduction. Bars represent means of viability relative to vector ± SEM from four experiments. Asterisks without brackets indicate statistically significant differences (p < 0.05) from vector control for the corresponding time point. Brackets indicate pairwise comparisons as shown. B. Relative dose dependence of cytotoxicity as measured by resazurin reduction. The dose of plasmids encoding each construct was diluted with vector as indicated while maintaining the total DNA at 500 ng. Symbols represent means of four experiments ± SEM. Lines are best fits to the Hill equation.