Table 1.
Effect of incubation on the diversity of bacterial and fungal communities associated with eggs.
| NS1 | OTUs2 | Estimated OTU richness |
Shannon index | ESC3 | ||
|---|---|---|---|---|---|---|
| ACE | Chao1 | |||||
| Bacteria | ||||||
| Early incubation | 92 | 37 | 167 (119; 244) | 137 (69; 348) | 3.16 (2.95; 3.38) | 0.185 |
| Late incubation | 95 | 19 | 59 (39; 101) | 64 (32; 167) | 2.35 (2.16; 2.55) | 0.406 |
| Fungi | ||||||
| Early incubation | 65 | 15 | 15 (15; 22) | 15 (15; 18) | 2.47 (2.30; 2.64) | 0.38 |
| Late incubation | 85 | 7 | 7 (7; 15) | 7 (7; 7) | 1.54 (1.38; 1.70) | 0.53 |
OTU, operational taxonomic unit. Data are based on sequencing of the 16S rRNA gene (bacteria) and ITS region (fungi) obtained from two clone libraries: early and late incubated pigeon eggs. Given values correspond to their average (lowest; highest values).
1
Number of sequences for each clone library.
2
Calculated Mothur at 99% of nucleotide identity (16S) and at 98% of nucleotide identity (ITS).
3
Estimated sample coverage: Cx = 1−(Nx/n), where Nx is the number of unique sequences, and n is the total number of sequences.