Table 1. Single-gel analysis of proteins that showed differences in abundance between 2-day-old WT and heterozygous or homozygous αA-R49C lenses.
| Spot number | Protein | UNIPROT accession number | MW (kDa) | Number of assigned spectra | Fold change | ||
| WT1 vs. WT2 | WT1 vs. heterozygous | WT2 vs. heterozygous | |||||
| 3040 | serum albumin | P07724 | 69 | 39 | 3.67 | 17.57 | 16.1 |
| αA-crystallin | Q569M7 | 20 | 5 | ||||
| Filensin | A2AMT1 | 74 | 3 | ||||
| 4024 | αA-crystallin | Q569M7 | 20 | 11 | 1.48 | 16.89 | 14.83 |
| Actin cytoplasmic | P62737 | 42 | 5 | ||||
| Creatine kinase B | Q04447 | 43 | 4 | ||||
| Erlin-2 | Q8BFZ9 | 38 | 3 | ||||
| 4090 | αA-crystallin | Q569M7 | 20 | 16 | 1.45 | 15.56 | 11.46 |
| Actin cytoplasmic | P62737 | 42 | 5 | ||||
| Creatine kinase B | Q04447 | 43 | 3 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 1 | ||||
| 4166 | αA-crystallin | Q569M7 | 20 | 1 (99%) | 1.39 | 15.09 | 10.67 |
| 4893 | Actin cytoplasmic | P62737 | 42 | 1 | 1.39 | 10.79 | 8.77 |
| Citron Rho-interacting kinase | P49025 | 235 | 1 | ||||
| 14-3-3 protein sigma | O70456 | 28 | 1 | ||||
| Peroxiredoxin-2 | Q61171 | 22 | 1 | ||||
| Glyoxalase domain-containing protein 4 | Q9CPV4 | 20 | 1 | ||||
| 5616 | αA-crystallin | Q569M7 | 20 | 21 | 1.24 | 7.51 | 7.46 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 17 | ||||
| βA2-crystallin | Q9QXC6 | 22 | 3 | ||||
| βA4-crystallin | Q3TSJ3 | 24 | 2 | ||||
| 5816 | αA-crystallin | Q569M7 | 20 | 19 | 1.22 | 6.2 | 6.91 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 5 | ||||
| γA-crystallin | Q6PGI0 | 21 | 3 | ||||
| γD-crystallin | P04345 | 21 | 2 | ||||
| 5909 | αA-crystallin | Q569M7 | 20 | 11 | 1.17 | 6 | 6.83 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 3 | ||||
| γA-crystallin | P04345 | 21 | 2 | ||||
| γD-crystallin | Q6PGI0 | 21 | 2 | ||||
| γB-crystallin | P04344 | 21 | 2 | ||||
| Eukaryotic trans initiation factor | P63242 | 17 | 1 | ||||
| 5955 | αA-crystallin | Q569M7 | 20 | 12 | 1.12 | 5.41 | 4.76 |
| γA-crystallin | P04345 | 21 | 2 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| γD-crystallin | Q6PGI0 | 21 | 1 | ||||
| 5976 | αA-crystallin | Q569M7 | 20 | 17 | 1.08 | 4.89 | 4.57 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| γA-crystallin | P04345 | 21 | 2 | ||||
| 6006 | αA-crystallin | Q569M7 | 20 | 14 | 1.08 | 3.17 | 3.68 |
| Eukaryotic trans initiation factor | P63242 | 17 | 1 | ||||
| 6037 | αA-crystallin | Q569M7 | 20 | 19 | −1.11 | 2.97 | 3.63 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| 6061 | αA-crystallin | Q569M7 | 20 | 37 | −1.13 | 2.62 | 2.5 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 1 | ||||
| γA-crystallin | P04345 | 21 | 1 | ||||
| 6176 | αA-crystallin | Q569M7 | 20 | 14 | −1.21 | 1.86 | 2.15 |
| Activated RNA polymerase II transcriptional coactivator p15 | P11031 | 14 | 4 | ||||
| 6218 | αA-crystallin | Q569M7 | 20 | 14 | −1.75 | 1.74 | −2.06 |
| Grifin | Q9D1U0 | 16 | 3 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| Spot number | Protein | UNIPROT accession number | MW (kDa) | Number of assigned spectra | Fold change | ||
| WT1 vs. WT2 | WT1 vs. homozygous | WT2 vs. homozygous | |||||
| 1462 | Spectrin-α | A3KGU5 | 283 | 16 | 1.09 | 10.15 | 9.34 |
| Nucleosome assembly protein 1-like 4 | Q792Z1 | 43 | 1 | ||||
| 1538 | Spectrin-α | A3KG45 | 283 | 59 | 1.21 | 8.33 | 6.91 |
| Neuronal cell adhesion molecule | Q810U4 | 139 | 8 | ||||
| αA-crystallin | Q569M7 | 20 | 5 | ||||
| Serrate RNA effector molecule homolog | Q99MR6 | 100 | 2 | ||||
| Methionine synthase | A6H5Y3 | 139 | 2 | ||||
| 2296 | Filensin | A2AMT1 | 74 | 43 | −1.07 | −5.61 | −5.19 |
| Gelsolin | P13020 | 86 | 5 | ||||
| Calpain 3 | A2AVV5 | 85 | 4 | ||||
| 3001 | 60 kDa Heat shock protein, mitochondrial | P63038 | 61 | 16 | −1.11 | 6.23 | 6.95 |
| T-complex protein 1 subunit theta | P42932 | 60 | 9 | ||||
| αA-crystallin | Q569M7 | 20 | 5 | ||||
| Tubulin alpha-1A chain | P68369 | 50 | 4 | ||||
| Tubulin beta-5 chain | P99024 | 50 | 2 | ||||
| Vimentin | P20152 | 54 | 1 | ||||
| Glutathione synthetase | P51855 | 52 | 1 | ||||
| 3084 | Ezrin | P26040 | 69 | 10 | 1.28 | 3.98 | 3.12 |
| Fascin | Q61553 | 55 | 5 | ||||
| αA-crystallin | Q569M7 | 20 | 5 | ||||
| D-3-phosphoglycerate dehydrogenase | Q61753 | 57 | 3 | ||||
| α-Enolase | P17182 | 47 | 2 | ||||
| βB1-crystallin | Q9WVJ5 | 28 | 2 | ||||
| Aspartyl-tRNA synthetase, cytoplasmic | Q922B2 | 57 | 1 | ||||
| 3295 | αA-crystallin | Q569M7 | 20 | 15 | 1.01 | −2.92 | −2.92 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 5 | ||||
| βA4-crystallin | Q9JJV0 | 22 | 4 | ||||
| γE-crystallin | QO3740 | 21 | 3 | ||||
| γB-crystallin | PO4344 | 21 | 2 | ||||
| Superoxide dismutase [Cu-Zn] | P08228 | 16 | 2 | ||||
| 3312 | αA-crystallin | Q569M7 | 20 | 23 | −1.12 | −5.51 | −4.9 |
| 3703 | αA-crystallin | Q569M7 | 20 | 13 | 1.01 | 14.68 | 14.6 |
| Filaggrin-2 | Q5D862 | 248 | 1 | ||||
| 3723 | αA-crystallin | Q569M7 | 20 | 14 | −1.58 | 2.99 | 4.75 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 3 | ||||
| βA4-crystallin | Q9JJV0 | 22 | 3 | ||||
| βB3-crystallin | Q9JJU9 | 24 | 1 | ||||
| Superoxide dismutase [Cu-Zn] | P08228 | 16 | 1 | ||||
| 3737 | αA-crystallin | Q569M7 | 20 | 14 | 1.09 | 4.9 | 4.53 |
| 3857 | αA-crystallin | Q569M7 | 20 | 15 | 1.09 | 31.67 | 29.19 |
| Grifin | Q9D1U0 | 16 | 1 | ||||
| 4133 | αA-crystallin | Q569M7 | 20 | 11 | 1.22 | 3.93 | 3.24 |
| Grifin | Q9D1U0 | 16 | 2 | ||||
| 4545 | αA-crystallin | Q569M7 | 20 | 11 | 1.13 | 6.68 | 5.93 |
| βA4-crystallin | Q9JJV0 | 22 | 2 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| 4932 | Fatty acid binding protein epidermal | Q05816 | 20 | 18 | −1.01 | 2.97 | 3.01 |
| αA-crystallin | Q569M7 | 20 | 8 | ||||
| βA4-crystallin | Q9JJV0 | 22 | 2 | ||||
| 5163 | αA-crystallin | Q569M7 | 20 | 5 | 1.00 | 4.26 | 4.28 |
| Fatty acid binding protein epidermal | Q05816 | 20 | 3 | ||||
| 5169 | βB3-crystallin | Q9JJU9 | 24 | 6 | 1.33 | 21.6 | 16.28 |
| αA-crystallin | Q569M7 | 20 | 4 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 1 | ||||
| 5182 | αA-crystallin | Q569M7 | 20 | 5 | −1.41 | 6.57 | 9.3 |
| βB3-crystallin | Q9JJU9 | 24 | 4 | ||||
| Calpain-3 | Q64691 | 94 | 1 | ||||
| βA3/A1-crystallin | Q9QCX6 | 25 | 1 | ||||
| 5223 | αA-crystallin | Q569M7 | 20 | 6 | −1.06 | −4.42 | −4.15 |
| 5247 | αA-crystallin | Q569M7 | 20 | 4 | −1.05 | 2.69 | 2.84 |
| βB3-crystallin | Q9JJU9 | 24 | 2 | ||||
| 5257 | αA-crystallin | Q569M7 | 20 | 3 | 1.05 | 3.33 | 3.21 |
| Thymosin beta-4 | P20065 | 6 | 1 | ||||
| 5441 | αA-crystallin | Q569M7 | 20 | 4 | 1.43 | 8.35 | 5.85 |
| 5639 | αA-crystallin | Q569M7 | 20 | 5 | −1.05 | 4.83 | 5.1 |
| 5675 | αA-crystallin | Q569M7 | 20 | 5 | 1.24 | 8.29 | 6.71 |
| Thymosin beta-4 | P20065 | 6 | 2 | ||||
| 5816 | αA-crystallin | Q569M7 | 20 | 7 | −1.21 | 6.45 | 7.83 |
| βA3/A1-crystallin | Q9QCX6 | 25 | 2 | ||||
| βA4-crystallin | Q9KKV0 | 22 | 2 | ||||
| 5883 | Glutamate dehydrogenase | P26443 | 61 | 3 | 1.24 | 4.01 | 3.26 |
| 5966 | Creatine kinase B-type | Q04447 | 43 | 8 | −1.1 | 19.82 | 22.03 |
| αA-crystallin | Q569M7 | 20 | 7 | ||||
| Actin cytoplasmic 1 | P60709 | 42 | 5 | ||||
| COP9 signalosome complex subunit 4 | O88544 | 46 | 4 | ||||
| 26 S proteasome non-ATPase regulatory subunit 13 | Q9WJJ2 | 43 | 3 | ||||
| Erlin-2 | Q8BFZ9 | 38 | 2 | ||||
| Activator of 90 kDa HSP ATPase homolog 1 | Q8BK64 | 38 | 2 | ||||
| Eukaryotic initiation factor 4A-I | P60843 | 46 | 1 | ||||
| Succinyl-CoA subunit beta | Q9Z2I9 | 38 | 1 | ||||
| Farnesyl pyrophosphate synthase | Q920E5 | 50 | 1 | ||||
| Glutaredoxin-3 | Q9CQM9 | 38 | 1 | ||||
| 5976 | βB3-crystallin | Q9JJU9 | 24 | 8 | −1.17 | 14.73 | 17.35 |
| αA-crystallin | Q569M7 | 20 | 6 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 3 | ||||
| βB2-crystallin | P62696 | 23 | 2 | ||||
| Gelsolin | P13020 | 86 | 2 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 1 | ||||
| γN-crystallin | Q8VHL5 | 21 | 1 | ||||
| 6042 | αA-crystallin | Q569M7 | 20 | 5 | 1.13 | 112.49 | 100.21 |
| βB3-crystallin | Q9JJU9 | 24 | 5 | ||||
| α-Enolase | P17182 | 47 | 3 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 2 | ||||
| βB1-crystallin | Q9WVJ5 | 28 | 2 | ||||
| Poly(γC)-binding protein 1 | P60335 | 37 | 2 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 1 | ||||
| γN-crystallin | Q8VHL5 | 21 | 1 | ||||
| 6051 | Glutamate dehydrogenase mitochondrial | P26443 | 61 | 4 | −1.27 | 3.73 | 4.75 |
| Myoglobin | P04249 | 17 | 1 | ||||
| 6224 | αA-crystallin | Q569M7 | 20 | 5 | 1.34 | 23.76 | 17.83 |
| βB3-crystallin | Q9JJU9 | 24 | 4 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 2 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 1 | ||||
| 6312 | αA-crystallin | Q569M7 | 20 | 7 | 1.13 | −2.72 | −3.04 |
| βB3-crystallin | Q9JJU9 | 24 | 2 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| 6457 | βB3-crystallin | Q9JJU9 | 24 | 18 | 1.19 | 5.94 | 5.03 |
| αA-crystallin | Q569M7 | 20 | 5 | ||||
| γB-crystallin | Q6PHP7 | 21 | 3 | ||||
| γF-crystallin | Q9CXV3 | 21 | 3 | ||||
| Triosephosphate isomerase | P17751 | 27 | 1 | ||||
| αB-crystallin | P23927 | 20 | 1 | ||||
| 6465 | βB1-crystallin | Q9WVJ5 | 28 | 18 | −1.01 | 3.29 | 3.35 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 5 | ||||
| Proteasome subunit 1 type 4 | P99026 | 29 | 5 | ||||
| αA-crystallin | Q569M7 | 20 | 4 | ||||
| βB3-crystallin | Q9JJU9 | 24 | 2 | ||||
| βA4-crystallin | Q9JJV0 | 22 | 2 | ||||
| βA2-crystalin | Q9JJV1 | 22 | 1 | ||||
| 6484 | αA-crystallin | Q569M7 | 20 | 6 | 1.34 | 6.74 | 5.05 |
| βB1-crystallin | Q9WVJ5 | 28 | 5 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 3 | ||||
| Proteasome subunit 1 type 4 | P99026 | 29 | 2 | ||||
| βB3-crystallin | Q9JJU9 | 24 | 1 | ||||
| 6546 | αA-crystallin | Q569M7 | 20 | 10 | 1.78 | 5.92 | 3.34 |
| βB3-crystallin | Q9JJU9 | 24 | 9 | ||||
| βB1-crystallin | Q9WVJ5 | 28 | 5 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 4 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 3 | ||||
| βS-crystallin | O35486 | 21 | 1 | ||||
| 6567 | αB-crystallin | P23927 | 20 | 17 | 1.54 | 9.13 | 5.96 |
| βB3-crystallin | Q9JJU9 | 24 | 8 | ||||
| αA-crystallin | Q569M7 | 20 | 5 | ||||
| βS-crystallin | O35486 | 21 | 5 | ||||
| γF-crystallin | Q9CXV3 | 21 | 4 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 3 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 1 | ||||
| 6605 | βA3/A1-crystallin | Q9QXC6 | 25 | 15 | −1.32 | 4.39 | 5.83 |
| βB3-crystallin | Q9JJU9 | 24 | 12 | ||||
| αB-crystallin | P23927 | 20 | 7 | ||||
| αA-crystallin | Q569M7 | 20 | 5 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 5 | ||||
| βS-crystallin | O35486 | 21 | 3 | ||||
| γF-crystallin | Q9CXV3 | 21 | 2 | ||||
| 6607 | αA-crystallin | Q569M7 | 20 | 12 | −1.04 | 2.82 | 2.95 |
| γB-crystallin | Q6PHP7 | 21 | 6 | ||||
| αB-crystallin | P23927 | 20 | 4 | ||||
| βB1-crystallin | Q9WVJ5 | 28 | 4 | ||||
| Calcium-regulated heat stable protein 1 | Q9CR86 | 16 | 3 | ||||
| γF-crystallin | Q9CXV3 | 21 | 2 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 1 | ||||
| βA4-crystallin | Q9JJV0 | 22 | 1 | ||||
| γC-crystalin | Q61597 | 21 | 1 | ||||
| 6642 | αA-crystallin | Q569M7 | 20 | 5 | −1.18 | 10.22 | 12.14 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 1 | ||||
| γB-crystallin | Q6PHP7 | 21 | 1 | ||||
| 6652 | αA-crystallin | Q569M7 | 20 | 7 | 1.17 | 9.1 | 7.79 |
| 6663 | αA-crystallin | Q569M7 | 20 | 6 | −1.21 | 6.79 | 8.29 |
| 6678 | Hemoglobin subunit α | P01942 | 15 | 1 | 1.34 | 11.01 | 8.26 |
| Glyceraldehyde-3-phosphate | P25856 | 42 | 1 | ||||
| Hemoglobin subunit β | P11758 | 16 | 1 | ||||
| 6791 | αA-crystallin | Q569M7 | 20 | 5 | 1.27 | 10.07 | 7.96 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 3 | ||||
| βB3-crystallin | Q9JJU9 | 24 | 2 | ||||
| 6920 | αA-crystallin | Q569M7 | 20 | 76 | −1.23 | 21.71 | 26.75 |
| 6981 | αA-crystallin | Q569M7 | 20 | 5 | −1.00 | 26.33 | 26.6 |
| γB-crystallin | Q6PHP7 | 21 | 1 | ||||
| Activated RNA polymerase II transcriptional coactivator P15 | P11031 | 14 | 1 | ||||
| Adenosine receptor A2B | Q60614 | 36 | 1 | ||||
| 7559 | αA-crystallin | Q569M7 | 20 | 15 | −2.29 | 8.38 | 19.32 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 5 | ||||
| Stathmin | P54227 | 17 | 4 | ||||
| βB3-crystallin | Q9JJU9 | 24 | 3 | ||||
| βA4-crystallin | Q9JJV0 | 22 | 2 | ||||
| Superoxide dismutase [Cu-Zn] | P08228 | 16 | 1 | ||||
| Fatty acid binding protein, epidermal | Q05816 | 15 | 1 | ||||
WT, wild-type.