Table 2. Multi-gel analysis of proteins that showed differences in abundance between 2-day-old WT and heterozygous or homozygous αA-R49C lenses.
| Spot number | Protein | UNIPROT Accession number | MW (kDa) | Number of assigned spectra | Fold change/t-test (p-value) | ||
| WT vs. heterozygous | WT vs. homozygous | Heterozygous/ homozygous | |||||
| 593 | Alanyl-tRNA synthetase, cytoplasmic | Q8BGQ7 | 107 | 12 | 2.01/0.035 | 2.28/0.021 | −1.13/0.43 |
| αA-crystallin | Q569M7 | 20 | 2 | ||||
| T-complex protein 1 subunit theta | P42932 | 60 | 2 | ||||
| Glutathione synthetase | P51855 | 52 | 2 | ||||
| 884 | βA3/A1-crystallin | Q9QXC6 | 25 | 1 | 1.02/0.83 | 1.65/0.017 | −1.62/0.0048 |
| Putative uncharacterized protein | Q3UAF6 | 42 | 1 | ||||
| 928 | Heat shock cognate 71 kDa protein | P63017 | 71 | 28 | 1.15/0.11 | 1.42/0.018 | −1.24/0.077 |
| V-type proton ATPase catalytic subunit | P50516 | 68 | 17 | ||||
| Stress-70 protein, mitochondrial | P38647 | 74 | 5 | ||||
| Alpha-fetoprotein | P02772 | 67 | 3 | ||||
| 948 | Stress-70 protein, mitochondrial | P38647 | 74 | 6 | 1.39/0.064 | 1.79/0.017 | −1.29/0.12 |
| αA-crystallin | Q569M7 | 20 | 4 | ||||
| Heat shock cognate 71 kDa protein | P63017 | 71 | 3 | ||||
| Glutathione synthetase | P51855 | 52 | 1 | ||||
| 1081 | T-complex protein 1 subunit theta | P42932 | 60 | 16 | 1.93/0.053 | 2.03/0.044 | −1.05/0.43 |
| αA-crystallin | Q569M7 | 20 | 4 | ||||
| 1131 | Seryl-tRNA synthetase, cytoplasmic | P26638 | 58 | 2 | 1.39/0.13 | 1.62/0.051 | −1.17/0.04 |
| 1146 | αA-crystallin | Q569M7 | 20 | 9 | 7.67/0.0037 | 10.91/0.0018 | −1.42/0.075 |
| T-complex protein 1 subunit theta | P42932 | 60 | 3 | ||||
| 1190 | βB1-crystallin | Q9WVJ5 | 28 | 1 | 1.1/0.22 | 1.28/0.013 | −1.16/0.029 |
| 1351 | Filensin | A2AMT1 | 74 | 6 | 1.26/0.006 | 1.22/0.014 | 1.03/0.68 |
| Eukaryotic translation initiation factor | P60229 | 52 | 3 | ||||
| 1477 | αA-crystallin | Q569M7 | 20 | 5 | −3.53/0.063 | −4.25/0.043 | 1.2/0.47 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| 1519 | Creatine kinase B- type | Q04447 | 43 | 11 | 15.85/0.00026 | 22.33/0.0001 | −1.41/0.14 |
| αA-crystallin | Q569M7 | 20 | 9 | ||||
| Putative uncharacterized protein | Q3UAF6 | 42 | 2 | ||||
| 1540 | αA-crystallin | Q569M7 | 20 | 11 | 53.41/6.9e-005 | 79.7/5.10E-05 | −1.49/0.077 |
| 1582 | αA-crystallin | Q569M7 | 20 | 13 | 10.11/0.0025 | 14.62/0.0014 | −1.45/0.098 |
| 1612 | αA-crystallin | Q569M7 | 20 | 9 | −2.77/0.12 | −4.82/0.041 | 1.74/0.19 |
| 1625 | αA-crystallin | Q569M7 | 20 | 1 | 2.75/0.031 | −1.42/0.21 | 3.9/0.01 |
| Fructose-biphosphate aldolase A | P05064 | 36 | 1 | ||||
| 1659 | αA-crystallin | Q569M7 | 20 | 12 | 1.44/0.0027 | 2.24/0.0069 | −1.56/0.12 |
| Eukaryotic translation initiation factor | Q9QZD9 | 36 | 2 | ||||
| Putative uncharacterized protein | Q3UAF6 | 42 | 1 | ||||
| 1767 | Glyceraldehyde-3-phosphate dehydrogenase | P16858 | 36 | 2 | −2.11/0.085 | −1.78/0.069 | −1.19/0.12 |
| Heterogeneous nuclear ribonucleoproteins A2/B1 | O88569 | 37 | 2 | ||||
| 1786 | Heterogeneous ribonucleoprotein A1 | P49312 | 34 | 5 | −1.93/0.053 | −1.16/0.42 | −1.67/0.088 |
| 1856 | βB1-crystallin | Q9WVJ5 | 28 | 8 | 1.08/0.54 | −1.65/0.089 | 1.77/0.0045 |
| 1865 | βB1-crystallin | Q9WVJ5 | 28 | 13 | −2.14/0.0017 | −1.42/0.075 | −1.51/0.057 |
| 1868 | βB1-crystallin | Q9WVJ5 | 28 | 8 | −1.04/0.47 | −1.48/0.049 | 1.42/0.12 |
| Eukaryotic translation initiation factor 4H | Q9WUK2 | 27 | 2 | ||||
| 1902 | βB1-crystallin | Q9WVJ5 | 28 | 12 | 1.06/0.50 | −2/0.0075 | 2.13/0.0022 |
| 2640 | αA-crystallin | Q569M7 | 20 | 9 | −2.15/0.00096 | −2.66/0.00022 | 1.24/0.036 |
| Histone H4 | P62806 | 11 | 2 | ||||
| Implantin (fragment) | P83891 | 2 | 1 | ||||
| 2661 | Regulating synaptic membrane exocytosis protein 2 | Q9EQZ7 | 173 | 1 | −2.38/0.039 | 1.00/0.95 | −2.39/0.021 |
| 2684 | Myotrophin | P62774 | 13 | 2 | −1.32/0.11 | −1.57/0.033 | 1.19/0.16 |
| 2772 | αA-crystallin | Q569M7 | 20 | 8 | −3.65/0.074 | −7.3/0.03 | 2/0.18 |
| βA4-crystallin | Q9JJV0 | 22 | 3 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| 1923 | βB1-crystallin | Q9WVJ5 | 28 | 2 | 1.43/0.053 | −1.54/0.019 | 2.2/0.011 |
| 2018 | αA-crystallin | Q569M7 | 20 | 8 | −1.4/0.021 | −1.13/0.35 | −1.24/0.059 |
| Implantin (fragment) | P83891 | 2 | 2 | ||||
| Coactosin-like protein | Q9CQI6 | 16 | 2 | ||||
| 2109 | βA3/A1-crystallin | Q9QXC6 | 25 | 12 | −1.16/0.18 | −1.49/0.034 | 1.28/0.15 |
| αA-crystallin | Q569M7 | 20 | 6 | ||||
| βA4-crystallin | Q9JJV0 | 22 | 5 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 2 | ||||
| 2115 | βA3/A1-crystallin | Q9QXC6 | 25 | 9 | −3.14/0.059 | −3.92/0.034 | 1.25/0.48 |
| αB-crystallin | P23296 | 20 | 9 | ||||
| βB2-crystallin | P62696 | 23 | 9 | ||||
| βB3-crystallin | Q9JJU9 | 24 | 8 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 8 | ||||
| αA-crystallin | Q569M7 | 20 | 6 | ||||
| 2123 | αA-crystallin | Q569M7 | 20 | 17 | −4.44/0.029 | −8.57/0.016 | 1.93/0.17 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 8 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 8 | ||||
| βB2-crystallin | P62696 | 23 | 6 | ||||
| αB-crystallin | P23296 | 20 | 3 | ||||
| βB3-crystallin | Q9JJU9 | 24 | 2 | ||||
| 2154 | αA-crystallin | Q569M7 | 20 | 12 | −1.44/0.0024 | −1.26/0.0094 | −1.14/0.13 |
| 2233 | αB-crystallin | P23296 | 20 | 19 | −1.59/0.084 | −1.8/0.053 | 1.13/0.35 |
| γD-crystallin | Q6PGI0 | 20 | 16 | ||||
| γA-crystallin | P04345 | 21 | 7 | ||||
| γF-crystallin | Q9CXV3 | 21 | 4 | ||||
| γB-crystallin | Q6PHP7 | 21 | 3 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| Plectin | Q9QXS1 | 534 | 2 | ||||
| 2261 | γA-crystallin | P04345 | 21 | 2 | 1.65/0.074 | −1.53/0.11 | 2.52/0.022 |
| αB-crystallin | P23296 | 20 | 2 | ||||
| 2294 | αA-crystallin | Q569M7 | 20 | 16 | 4.16/0.020 | 5.64/0.012 | −1.36/0.054 |
| 2317 | αA-crystallin | Q569M7 | 20 | 14 | −2.83/0.095 | −5.09/0.032 | 1.8/0.17 |
| 2320 | αA-crystallin | Q569M7 | 20 | 21 | −4.85/0.11 | −9.28/0.035 | 1.91/0.14 |
| 2337 | βA3/A1-crystallin | Q9QXC6 | 25 | 8 | −2.28/0.024 | −3.49/0.06 | 1.53/0.23 |
| αA-crystallin | Q569M7 | 20 | 8 | ||||
| βA2-crystallin | Q9JJV1 | 22 | 2 | ||||
| 2351 | αA-crystallin | Q569M7 | 20 | 12 | 4.61/0.0057 | 10.45/0.0049 | −2.27/0.14 |
| 2388 | αA-crystallin | Q569M7 | 20 | 10 | 1.48/0.27 | 5.71/0.038 | −3.85/0.074 |
| 2413 | γD-crystallin | Q6PGI0 | 20 | 10 | −1.14/0.86 | −3.8/0.046 | 3.33/0.007 |
| Peptidyl-prolyl cis-trans isomerase | P17742 | 18 | 6 | ||||
| γA-crystallin | P04345 | 21 | 6 | ||||
| γB-crystallin | Q6PHP7 | 21 | 4 | ||||
| γC-crystallin | Q61597 | 21 | 4 | ||||
| 2417 | αA-crystallin | Q569M7 | 20 | 15 | 7.51/0.0023 | 10.53/0.0015 | −1.4/0.15 |
| 2454 | Nucleoside diphosphate kinase | E9PZF0 | 30 | 6 | −1.25/0.016 | −1.47/0.0067 | 1.18/0.11 |
| Peptidyl-prolyl cis-trans isomerase | P17742 | 18 | 4 | ||||
| γD-crystallin | Q6PGI0 | 20 | 3 | ||||
| αA-crystallin | Q569M7 | 20 | 3 | ||||
| γB-crystallin | Q6PHP7 | 21 | 2 | ||||
| 2462 | αA-crystallin | Q569M7 | 20 | 3 | −1.48/0.12 | −2.08/0.017 | 1.41/0.021 |
| γN-crystallin | Q8VHL5 | 21 | 2 | ||||
| 2469 | Nucleoside diphosphate kinase | E9PZF0 | 30 | 6 | −1.37/0.052 | −1.56/0.023 | 1.14/0.17 |
| αA-crystallin | Q569M7 | 20 | 3 | ||||
| 2501 | γC-crystallin | Q61597 | 21 | 2 | −1.55 | −2.11/0.012 | 1.36 |
| 2533 | αA-crystallin | Q569M7 | 20 | 3 | −1.82/0.10 | −2.44/0.027 | 1.34/0.26 |
| 2538 | αA-crystallin | Q569M7 | 20 | 3 | −2.41/0.021 | −2.32/0.067 | −1.04/0.88 |
| 40S ribosomal protein S12 | P63323 | 15 | 2 | ||||
| 2553 | Fatty acid-binding protein epidermal | Q05816 | 15 | 12 | −1.96/0.67 | −3.48/0.2 | 1.77/0.19 |
| αA-crystallin | Q569M7 | 20 | 7 | ||||
| 2631 | αA-crystallin | Q569M7 | 20 | 2 | −2.58/0.13 | −2.92/0.038 | 1.13/0.079 |
WT, wild-type.