Table 3. Protein spots that showed a change in abundance between 2-week-old WT and αA-R49C homozygous mutant lenses.
| Spot number | Protein | Uniprot accession number | MW (kDa) | Number of assigned spectra | Fold change | ||
| WT1 vs. WT2 | WT1 vs. homozygous | WT2 vs. homozygous | |||||
| 700 | Spectrin α-2 | A3KGU5 | 283 | 47 | −1.6 | −13.77 | −8.51 |
| 769 | Spectrin α-2 | A3KGU5 | 283 | 20 | 1.77 | −8.19 | −14.35 |
| γA-crystallin | 21 | 2 | |||||
| 1164 | Adult male sm. intestine cDNA | Q9CPN9 | 26 | 1 | −2.56 | −7.87 | −3.05 |
| 2448 | Filensin | A2AMT1 | 74 | 39 | −2.05 | −15.14 | −7.32 |
| 2675 | Filensin | A2AMT1 | 74 | 8 | −1.89 | 3.81 | 7.27 |
| 78 kDa glucose-regulated protein precursor | P20029 | 72 | 4 | ||||
| GTP binding protein Di- Ras 1 | Q91Z61 | 22 | 1 | ||||
| Collagen α2v chain precursor | Q3U962 | 145 | 1 | ||||
| 2676 | 78 kDa glucose-regulated protein precursor | P20029 | 72 | 12 | −1.87 | 3.4 | 6.42 |
| 3641 | Phakinin | Q6NVD9 | 46 | 24 | −1.59 | −10.22 | −6.38 |
| 3880 | Phakinin | Q6NVD9 | 46 | 15 | 1.23 | 8.33 | 6.82 |
| 16 days embryo kidney cDNA | Q3TGJ9 | 81 | 7 | ||||
| 4035 | αA-crystallin | Q569M7 | 20 | 8 | 1.00 | 7.71 | 7.76 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 4 | ||||
| βB1-crystallin | Q9WVJ5 | 28 | 2 | ||||
| βB3-crystallin | Q9JJU9 | 24 | 2 | ||||
| 4058 | βA3/A1-crystallin | Q9QXC6 | 25 | 7 | 1.77 | 3.79 | 2.16 |
| αA-crystallin | Q569M7 | 20 | 4 | ||||
| βB1-crystallin | Q9WVJ5 | 28 | 1 | ||||
| Fructose-bisphosphate aldolase | A6ZI44 | 45 | 1 | ||||
| 4101 | αA-crystallin | Q569M7 | 20 | 8 | 1.2 | 3.41 | 2.86 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 6 | ||||
| βB3-crystallin | Q9JJU9 | 24 | 2 | ||||
| βB1-crystallin | Q9WVJ5 | 28 | 1 | ||||
| 4111 | αA-crystallin | Q569M7 | 20 | 3 | −1.49 | −10.27 | −6.85 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 3 | ||||
| βB3-crystallin | Q9JJU9 | 24 | 2 | ||||
| 40S ribosomal protein SA(Laminin receptor 1) | P14206 | 33 | 1 | ||||
| 4191 | αA-crystallin | Q569M7 | 20 | 7 | −1.07 | 26.32 | 28.36 |
| βB1-crystallin | Q9WVJ5 | 28 | 2 | ||||
| 4207 | Phakinin | Q6NVD9 | 46 | 8 | 2.02 | −9.58 | −19.19 |
| αA-crystallin | Q569M7 | 20 | 1 | ||||
| 4217 | αA-crystallin | Q569M7 | 20 | 10 | 1.36 | 5.67 | 4.21 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 7 | ||||
| βB1-crystallin | Q9WVJ5 | 28 | 2 | ||||
| Phakinin | Q6NVD9 | 46 | 2 | ||||
| 4627 | αA-crystallin | Q569M7 | 20 | 8 | 1.21 | 22.44 | 18.67 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| Adult male stomach cDNA | Q3Y2E0 | 27 | 2 | ||||
| Adult male sm. intestine cDNA | Q9CPN9 | 26 | 1 | ||||
| WD repeat-containing protein 81 | Q5ND34 | 96 | 1 | ||||
| NOD-derived CD11c positive dendritic cells cDNA | Q5ND34 | 17 | 1 | ||||
| 4872 | αA-crystallin | Q569M7 | 20 | 5 | −1.31 | 3.99 | 5.26 |
| Annexin A5 | ANXA5 | 36 | 5 | ||||
| Adult male stomach cDNA | Q3Y2E0 | 27 | 2 | ||||
| Adult male small intestine cDNA | Q9CPN9 | 26 | 1 | ||||
| SRC kinase signaling inhibitor 1 | Q9QWI6 | 135 | 1 | ||||
| Filamin-B | Q80X90 | 278 | 1 | ||||
| Probable E3 ubiquitin-protein ligase HERC2 | Q4U2R1 | 528 | 1 | ||||
| 5218 | βB1-crystallin | Q9WVJ5 | 28 | 13 | 1.76 | −24.55 | −42.86 |
| 5225 | βB1-crystallin | Q9WVJ5 | 28 | 20 | −1.05 | −6.09 | −5.72 |
| 5236 | βB1-crystallin | Q9WVJ5 | 28 | 20 | −1.27 | −8.76 | −6.82 |
| Complement factor C2 | Q792Q3 | 53 | 1 | ||||
| 5608 | βB1-crystallin | Q9WVJ5 | 28 | 15 | −1.23 | −8.75 | −7.05 |
| αA-crystallin | Q569M7 | 20 | 1 | ||||
| 5609 | βB3-crystallin | Q9JJU9 | 24 | 19 | 1.43 | 21.18 | 14.97 |
| Glutathione S-transferase µ | A2AE89 | 24 | 5 | ||||
| βB2-crystallin | P62696 | 23 | 3 | ||||
| βB1-crystallin | Q9WVJ5 | 28 | 1 | ||||
| Complement factor C2 | Q792Q3 | 53 | 1 | ||||
| Adult male sm. intestine cDNA | Q9CPN9 | 26 | 1 | ||||
| 5617 | βB2-crystallin | P62696 | 23 | 34 | −1.55 | −8.44 | −5.38 |
| βB3-crystallin | Q9JJU9 | 24 | 12 | ||||
| Glutathione S-transferase µ | A2AE89 | 24 | 5 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| βB1-crystallin | Q9WVJ5 | 28 | 1 | ||||
| 5625 | βB2-crystallin | P62696 | 23 | 17 | −1.38 | −18.58 | −13.36 |
| βB3-crystallin | Q9JJU9 | 24 | 6 | ||||
| Heat shock protein B1 (HspB1) | P14602 | 23 | 2 | ||||
| Glutathione S-transferase µ | A2AE89 | 24 | 2 | ||||
| NOD-derived Cd11c-positive dendritic cells cDNA | Q3TCH2 | 25 | 2 | ||||
| 5695 | βA3/A1-crystallin | Q9QXC6 | 25 | 66 | −1.53 | −20.2 | −13.04 |
| βB2-crystallin | P62696 | 23 | 56 | ||||
| βB3-crystallin | CRBB3 | 24 | 13 | ||||
| START domain containing 9 | AZAKH9 | 408 | 1 | ||||
| 5719 | βB2-crystallin | P62696 | 23 | 46 | 1.23 | 8.38 | 6.87 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 36 | ||||
| βB3-crystallin | CRBB3 | 24 | 32 | ||||
| βB1-crystallin | CRBB1 | 28 | 10 | ||||
| αA-crystallin | Q569M7 | 20 | 4 | ||||
| 5753 | βB3-crystallin | CRBB3 | 24 | 67 | 1.49 | 9.82 | 6.67 |
| βB2-crystallin | P62696 | 23 | 29 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 9 | ||||
| 5789 | βB3-crystallin | CRBB3 | 24 | 40 | 1.02 | 17.66 | 17.52 |
| βB1-crystallin | CRBB1 | 28 | 21 | ||||
| βB2-crystallin | P62696 | 23 | 6 | ||||
| αA-crystallin | Q569M7 | 20 | 4 | ||||
| Development and differentiation enhancing factor 2 | Q7SIG6 | 107 | 3 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 2 | ||||
| 5799 | βB1-crystallin | CRBB1 | 28 | 38 | −1.1 | 13.89 | 15.37 |
| βA3/A1-crystallin | Q9QXC6 | 25 | 11 | ||||
| Proteasome subunit beta type 4 | P99026 | 29 | 6 | ||||
| αA-crystallin | Q569M7 | 20 | 3 | ||||
| βA4-crystallin | Q9JJV0 | 22 | 3 | ||||
| Development and differentiation enhancing factor 2 | Q7SIG6 | 107 | 2 | ||||
| Adult male small intestine cDNA | Q9CPN9 | 26 | 2 | ||||
| Prickle3 protein | Q8CIL5 | 72 | 1 | ||||
| βB3-crystallin | CRBB3 | 24 | 1 | ||||
| Cardiotrophin-like cytokine factor 1 precursor | Q9QZM3 | 25 | 1 | ||||
| βA2-crystallin | CRBA2 | 22 | 1 | ||||
| UTP14, U3 small nucleolar ribonucleoprotein homolog A yeast | Q4QY64 | 87 | 1 | ||||
| Leucine-rich repeat-containing protein 33 precursor | Q8BMT4 | 77 | 1 | ||||
| Adult male testis cDNA RIKEN | Q9D9G7 | 28 | 1 | ||||
| GTP-binding protein Di-Ras1 | Q91Z61 | 22 | 1 | ||||
| NOD-derived CD11c positive dendritic cells cDNA | Q8VDD8 | 55 | 1 | ||||
| 16 days neonate thymus cDNA | Q3TRH2 | 48 | 1 | ||||
| Bone marrow macrophage cDNA | Q3UAB1_MOUSE | 50 | 1 | ||||
| DNA polymerase epsilon subunit 2 | DPOE2_MOUSE | 59 | 1 | ||||
| 5830 | βB1-crystallin | CRBB1 | 28 | 29 | 1.19 | −8.59 | −10.09 |
| βS (γS-crystallin) | O35486 | 21 | 15 | ||||
| γC-crystallin | Q61597 | 21 | 14 | ||||
| γB-crystallin | Q6PHP7 | 21 | 10 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 4 | ||||
| γD-crystallin | Q6PGI0 | 21 | 4 | ||||
| Glutathione S-transferase P1 | P19157 | 24 | 3 | ||||
| αA-crystallin | Q569M7 | 20 | 2 | ||||
| 6012 | αB-crystallin | P23927 | 20 | 42 | 1.07 | 14.08 | 13.3 |
| γB-crystallin | Q6PHP7 | 21 | 29 | ||||
| βA2-crystallin | CRBA2 | 22 | 25 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 23 | ||||
| βS (γS-crystallin) | O35486 | 21 | 20 | ||||
| γN-crystallin | Q8VHL5 | 21 | 18 | ||||
| αA-crystallin | Q569M7 | 20 | 15 | ||||
| γA-crystallin | P04345 | 21 | 8 | ||||
| γD-crystallin | Q6PGI0 | 21 | 7 | ||||
| γC-crystallin | Q61597 | 21 | 3 | ||||
| 6117 | αB-crystallin | P23927 | 20 | 43 | −1.14 | 4.51 | 5.17 |
| γB-crystallin | Q6PHP7 | 21 | 24 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 17 | ||||
| βA2-crystallin | CRBA2 | 22 | 14 | ||||
| γA-crystallin | P04345 | 21 | 11 | ||||
| γD-crystallin | Q6PGI0 | 21 | 10 | ||||
| γS-crystallin | O35486 | 21 | 7 | ||||
| αA-crystallin | Q569M7 | 20 | 4 | ||||
| γN-crystallin | Q8VHL5 | 21 | 4 | ||||
| γC-crystallin | Q61597 | 21 | 3 | ||||
| γE-crystallin | A2RTH4 | 21 | 3 | ||||
| Sodium channel voltage-gated Type III alpha | A2ASI5 | 221 | 1 | ||||
| 6161 | αB-crystallin | P23927 | 20 | 30 | 1.61 | 10.3 | 6.45 |
| αA-crystallin | Q569M7 | 20 | 19 | ||||
| γB-crystallin | Q6PHP7 | 21 | 6 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 6 | ||||
| γD-crystallin | Q6PGI0 | 21 | 5 | ||||
| βA2-crystallin | CRBA2 | 22 | 4 | ||||
| γA-crystallin | P04345 | 21 | 3 | ||||
| Tenascin precursor | Q80YX1 | 232 | 2 | ||||
| Mark 1 protein | Q14DQ3 | 88 | 1 | ||||
| Tenascin precursor | Q80YX1 | 232 | 1 | ||||
| 6341 | αA-crystallin | Q569M7 | 20 | 31 | 1.2 | −60.51 | −71.83 |
| αB-crystallin | P23927 | 20 | 9 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 6 | ||||
| βA2-crystallin | CRBA2 | 22 | 5 | ||||
| γN-crystallin | Q8VHL5 | 21 | 5 | ||||
| βB1-crystallin | CRBB1 | 28 | 4 | ||||
| βA4-crystallin | Q9JJV0 | 22 | 3 | ||||
| γA-crystallin | P04345 | 21 | 2 | ||||
| 6352 | αA-crystallin | Q569M7 | 20 | 25 | 1.2 | −13.31 | −15.81 |
| αB-crystallin | P23927 | 20 | 14 | ||||
| βA3/A1-crystallin | Q9QXC6 | 25 | 7 | ||||
| Adult male small intestine cDNA | Q9CPN9 | 26 | 3 | ||||
| Cardiotrophin-like cytokine factor 1 precursor | Q9QZM3 | 25 | 3 | ||||
| Leucine-rich repeat-containing protein 33 precursor | Q8BMT4 | 77 | 2 | ||||
| ELMO domain-containing protein 1 | Q3V1U8 | 38 | 2 | ||||
| γA-crystallin | P04345 | 21 | 1 | ||||
| UTP14, U3 small nucleolar ribonucleoprotein homolog A yeast | Q4QY64 | 87 | 1 | ||||
| βA2-crystallin | CRBA2 | 22 | 1 | ||||
| Adult male testis cDNA RIKEN | Q9D9G7 | 28 | 1 | ||||
| GTP-binding protein Di-Ras 1 | Q91Z61 | 22 | 1 | ||||
| Regulator of nonsense transcripts 1 | Q9EPU0 | 124 | 1 | ||||
| 6485 | αA-crystallin | Q569M7 | 20 | 26 | 1.35 | 34.08 | 25.47 |
| Adult male small intestine cDNA | Q9CPN9 | 26 | 2 | ||||
| αB-crystallin | P23927 | 20 | 2 | ||||
| Adult male testis cDNA RIKEN | Q9D9G7 | 28 | 2 | ||||
| γA-crystallin | P04345 | 21 | 1 | ||||
| Pyruvate carboxylase, mitochondrial | Q05920 | 130 | 1 | ||||
| GTP-binding protein Di-Ras 1 | Q91Z61 | 22 | 1 | ||||
| ATPase family AAA domain-containing protein 5 (chromosome fragility-associated gene 1 protein | Q4QY64 | 204 | 1 | ||||
| MKIAA0327 splice variant | Q8CHE5 | 102 | 1 | ||||
| 6520 | γA-crystallin | P04345 | 21 | 32 | 1.22 | −5.96 | −7.23 |
| γB-crystallin | Q6PHP7 | 21 | 22 | ||||
| γC-crystallin | A3RLD4 | 21 | 19 | ||||
| γD-crystallin | Q6PGI0 | 21 | 8 | ||||
| Adult male small intestine cDNA | Q9CPN9 | 26 | 1 | ||||
| 6663 | αA-crystallin | Q569M7 | 20 | 35 | 1.75 | 15.24 | 8.8 |
| γC-crystallin | A3RLD4 | 21 | 11 | ||||
| γA-crystallin | P04345 | 21 | 8 | ||||
| γB-crystallin | Q6PHP7 | 21 | 7 | ||||
| 2 days neonate thymus thymic cells cDNA | Q7TNP7 | 69 | 3 | ||||
| Adult male small intestine cDNA | Q9CPN9 | 26 | 2 | ||||
| γD-crystallin | Q6PGI0 | 21 | 2 | ||||
| Nuclear protein localization protein 4 homolog | P60670 | 68 | 2 | ||||
| 6668 | αA-crystallin | Q569M7 | 20 | 28 | 1.72 | 73.82 | 43.31 |
| Adult male small intestine cDNA | Q9CPN9 | 26 | 2 | ||||
| 2 days neonate thymus thymic cells cDNA | Q7TNP7 | 69 | 2 | ||||
| Nuclear protein localization protein 4 homolog | P60670 | 68 | 2 | ||||
| Adult male testis cDNA RIKEN | Q9D9G7 | 28 | 2 | ||||
| ELMO domain-containing protein 1 | Q3V1U8 | 38 | 2 | ||||
| Prickle3 protein | Q8CIL5 | 72 | 2 | ||||
| 6788 | αA-crystallin and many others | Q569M7 | 20 | 30 | 2.57 | 498.57 | 195.82 |
| Nuclear protein localization protein 4 homolog | P60670 | 68 | 3 | ||||
| 2 days neonate thymus thymic cells cDNA | Q7TNP7 | 69 | 2 | ||||
| 7068 | αA-crystallin | Q569M7 | 20 | 27 | −1.05 | −21.14 | −20.03 |
| 2 days neonate thymus thymic cells cDNA | Q7TNP7 | 69 | 2 | ||||
| Prickle3 protein | Q8CIL5 | 72 | 2 | ||||
| γA-crystallin | P04345 | 21 | 2 | ||||
| 7089 | γS-crystallin | O35486 | 21 | 14 | 1.76 | −8.63 | −15.01 |
| γC-crystallin | A3RLD4 | 21 | 8 | ||||
| γB-crystallin | Q6PHP7 | 21 | 3 | ||||
| αA-crystallin | Q569M7 | 20 | 2 | ||||
| γD-crystallin | Q6PGI0 | 21 | 1 | ||||
| 7269 | αA-crystallin | Q569M7 | 20 | 22 | 1.1 | 55.72 | 51.25 |
| UTP14, U3 small nucleolar ribonucleoprotein homolog A yeast | Q4QY64 | 87 | 2 | ||||
| Palmitoyl protein thioesterase-like protein | Q8R2F8 | 16 | 1 | ||||
| 7419 | αA-crystallin | Q569M7 | 20 | 18 | −2.33 | 14.04 | 32.99 |
| UTP14, U3 small nucleolar ribonucleoprotein homolog A yeast | Q4QY64 | 87 | 2 | ||||
| 2 days neonate thymus thymic cells cDNA | Q7TNP7 | 69 | 2 | ||||
| Adult male small intestine cDNA | Q9CPN9 | 26 | 2 | ||||
| 7540 | αA-crystallin | Q569M7 | 20 | 20 | 1.02 | 91.8 | 91.26 |
| Ceruloplasmin precursor | Q61147 | 121 | 8 | ||||
| Heparin cofactor 2 precursor | HEP2_mouse | 54 | 4 | ||||
| Plexin-A4 precursor | Q80UG2 | 213 | 3 | ||||
| Attractin | Q9WU60 | 158 | 3 | ||||
| Serum Amyloid-P component | Q4JFI8 | 26 | 3 | ||||
| Lumican precursor | P51885 | 38 | 2 | ||||
| Gelsolin | P13020 | 86 | 2 | ||||
| UTP14, U3 small nucleolar ribonucleoprotein homolog A yeast | Q4QY64 | 87 | 2 | ||||
| ELMO domain-containing protein 1 | Q3V1U8 | 38 | 2 | ||||
| Complement factor I precursor | Q61129 | 67 | 2 | ||||
| 7568 | αA-crystallin | Q569M7 | 20 | 17 | −1.45 | 134.39 | 197.02 |
| Ceruloplasmin precursor | Q61147 | 121 | 9 | ||||
| Gelsolin | P13020 | 86 | 4 | ||||
| Lumican precursor | P51885 | 38 | 4 | ||||
| Pyruvate carboxylase, mitochondrial | Q05920 | 130 | 3 | ||||
| Plexin-A4 precursor | Q80UG2 | 213 | 3 | ||||
| ELMO domain-containing protein 1 | Q3V1U8 | 38 | 3 | ||||
| Complement factor I precursor | Q61129 | 67 | 3 | ||||
| Cardiotrophin-like cytokine factor 1 precursor | Q9QZM3 | 25 | 2 | ||||
| GTP-binding protein Di-Ras 1 | Q91Z61 | 22 | 2 | ||||
| Myoferlin | Q69ZN7 | 233 | 2 | ||||
| 7751 | αA-crystallin | Q569M7 | 20 | 9 | 1.15 | 160.65 | 141.07 |
| Ceruloplasmin precursor | Q61147 | 121 | 8 | ||||
| Lumican precursor | P51885 | 38 | 4 | ||||
| ELMO domain-containing protein 1 | Q3V1U8 | 38 | 2 | ||||
| Cardiotrophin-like cytokine factor 1 precursor | Q9QZM3 | 25 | 2 | ||||
| 8192 | αA-crystallin | Q569M7 | 20 | 7 | 1.24 | 75.08 | 61.08 |
| Ceruloplasmin precursor | Q61147 | 121 | 7 | ||||
| Lumican precursor | P51885 | 38 | 3 | ||||
| ELMO domain-containing protein 1 | Q3V1U8 | 38 | 3 | ||||
| Adult male testis cDNA RIKEN | Q9D9G7 | 28 | 3 | ||||
| Adult male small intestine cDNA | Q9CPN9 | 26 | 2 | ||||
| UTP14, U3 small nucleolar ribonucleoprotein homolog A yeast | Q4QY64 | 87 | 2 | ||||
WT, wild-type.