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. 2014 Apr 23;9(4):e95102. doi: 10.1371/journal.pone.0095102

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© 2014 Xu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) SDS-PAGE analysis of the purified recombinant VpPR-10.1 protein. Lane 1, protein marker; lane 2, purified wild-type recombinant VpPR-10.1 protein; lane 3, purified recombinant Y151H mutant protein. (b) Lane 1, purified recombinant K55N mutant protein; lane 2, purified recombinant E149G mutant protein; lane 3, protein marker. VpPR10.1 and its mutant constructs in E. coli BL21 (DE3) were induced with 0.1 mM IPTG at 37 °C for 4 h, the gel was stained with Coomassie Blue R-250. (c) SDS-PAGE analysis of the VpPR-10.1 protein without GST. Lane 1, protein marker; lane 2, VpPR-10.1 protein without GST; lane 3, K55N protein without GST; lane 4, E149G protein without GST; lane 5, Y151H protein without GST. The VpPR-10.1 protein product, after GST digestion, was estimated to be approximately 17 kDa.