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. 2014 Apr 23;9(4):e95102. doi: 10.1371/journal.pone.0095102

Figure 4. Ribonuclease activities of VpPR-10.1 and mutants assayed on grapevine total RNA.

Figure 4

Samples with each recombinant VpPR-10.1 protein and grapevine total RNA in the presence of RNasin were incubated at 37 °C for 30 min. (a) Boiled proteins without GST purified from pGEX-4 T-1 in E. coli and elution buffer in the presence of RNasin were used as negative controls. (b) Recombinant proteins VpPR-10.1, Y151H, K55N, and E149G without GST were incubated with grapevine total RNA in the presence of RNasin. Mutant proteins K55N and E149G lost the function of degrading RNA. Elution buffer was used as a negative control.