Figure 6. Antifungal activity assays of VpPR-10.1 toward A. alternate.

(a) A. alternate was grown on PDB medium in the presence of purified wild-type recombinant VpPR-10.1 and evaluated after incubating for 5 days at room temperature. CK, oxidized glutathione buffer (the protein elution buffer) was used as qa negative control; WT-1, 20 µg of VpPR-10.1; WT-2, 40 µg of VpPR-10.1; WT-3, 60 µg of VpPR-10.1; WT-4, 80 µg of VpPR-10.1. (b) Analysis of A. alternate grown on PDB medium in the presence of purified wild-type recombinant VpPR-10.1 and mutant proteins at 80 µg·mL⁻¹. (c) A. alternate grown on PDB medium in the presence of 80 µg·mL⁻¹ purified wild-type recombinant VpPR-10.1 and mutant proteins were collected and diluted into 5 ml distilled water, then estimated by observing the absorbance at 595 nm. Each point on the plot is the average of three independent determinations.