Figure 2. Long- and short-shafted chimeric fibers with genetic insertion of the YSA peptide into the HAdV-41 short fiber knob: trimerization, incorporation into virus particles, and viral transduction.

(A) Immunoblot of unboiled cell lysates after transient transfection of fiber expression plasmids into 293T cells. (B) Immunoblot of purified virus particles of pseudotyped LacZ reporter viruses generated by the transfection/superinfection protocol. (C) Transduction of EphA2-positive and EphA2-negative cells with pseudotyped LacZ reporter viruses. SK-MEL-28 cells were transduced in quadruplicates, all other cell lines were transduced in triplicates. Columns and error bars show mean values and standard deviations of β-Gal activity, respectively. RLU, relative luminescence units; * p<0.05, ** p<0.01, *** p<0.001 versus 5T41sSK in the respective cell line.