Figure 4. Fibers with genetic insertion of the YSA peptide into the CAR binding-ablated HAdV-5 knob: trimerization, incorporation into virus particles, and transduction.

(A) Immunoblot of unboiled cell lysates after transient transfection of fiber expression plasmids into 293T cells. (B) Immunoblot of purified virus particles of pseudotyped LacZ reporter viruses. (C) Transduction of EphA2-positive and EphA2-negative cells with pseudotyped LacZ reporter viruses. Cells were transduced in quadruplicates, columns and error bars show mean values and standard deviations of β-Gal activity, respectively. RLU, relative luminescence units; *^/#p<0.05, **^/##p<0.01, ***^/###p<0.001 versus 5T/41sSK (*) or 5KO1 (^#).