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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Nat Struct Mol Biol. 2014 Apr 6;21(5):449–455. doi: 10.1038/nsmb.2810

Figure 5.

Figure 5

Mediator occupancy shifts towards core promoters in the absence of CTD Ser5 phosphorylation. (a) Average Mediator (Gal11, green) and RNAPII (Rpb1-Flag, red) occupancy on all genes with Rpb1-Flag average ORF occupancy >1 in the absence of rapamycin (n=345) in cells expressing wild type (WT, solid traces), S5A (dotted traces) and S7A (dashed traces) Rpb1 proteins, 90 minutes after nuclear depletion of the endogenous Rpb1 protein by CAND. TFIIB (purple) from WT cells is shown as a placeholder for core promoters. (b) Genome browser view of a region around the PMA1 gene. The vertical dashed line indicates PIC position and black arrows point toward Mediator accumulation at the promoter and RNAPII pile up in the 5′ end in the S5A mutant.