Table 1. Summary of macroscopic binding constants and thermodynamic parameters obtained from the ITC studies of Ca²⁺-binding isotherm of EhCaBPs at 25°C.

Ligand	Titrand	No of experimental Ca2+- binding sites (n)	KA (M-1)	Kd	ΔH (cal/mol)	ΔS (cal/mol)	ΔG (kcal/mol)
Ca2+	EhCaBP1	4	K1 = 5.25×103±4.0×102	130.72 µM	−1860±0	10.8	−4.84
			K2 = 1.41×104±9.5×102		2.3×105±0	790	−4.6×102
			K3 = 5.10×105±2.8×104		2.4×105±1.82×103	−780	−7.56
			K4 = 1.55×106±7.3×104		−7981±1.86×103	1.56	−8.44
	EhCaBP3	2	K1 = 4.00×106±5.3×105	1.85 µM	−1.605×104±86.6	−23.6	−9.0
			K2 = 7.28×104±5.3×103		−7573±104	−3.16	−6.63
	EhCaBP5	2	K = 1.18×107±1.47×106	85 nM	−1.84×104±61.79	−29.4	−9.64
	EhCaBP6	2	K1 = 1.07×105±1.1×104	46 µM	702±17.6	25.4	−6.86
			K2 = 4.44×103±1.1×102		5244±45.9	34.3	−4.97
	EhCaBP7	2	K1 = 1.04×106±2.5×105	3.12 µM	−1807±96.5	21.5	−8.2
			K2 = 9.86×104±6.8×103		−5413±96.5	4.69	−6.81