Table 1.
Antibody | Immunogen | Manufacturer | Dilution |
---|---|---|---|
class III β-tubulin (TUJl) |
microtubules derived from rat brain, reactive to neuron specific Class III β-tubulin (βIII) |
Covance, MMS-435P | 1:200 |
peripherin | Electrophoretically pure trp- E-peripherin fusion protein, containing all but the 4 N- terminal amino acids of rat peripherin. |
Chemicon, MAB1527 Raised in mouse Monoclonal antibody |
Basal neurons 1:4000; Middle neurons 1:5000; Apical neurons 1:6000 |
calretinin | Recombinant rat calretinin | Millipore, AB5054 Raised in rabbit Polyclonal antibody |
1:200 |
calretinin | Recombinant rat calretinin | Millipore, MAB1568 Raised in mouse Monoclonal antibody |
1:100 |
calbindin | Recombinant rat calbindin D-28k (CB) |
Swant, CB-38a Raised in rabbit Polyclonal antibody |
1:100 |