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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Neurochem. 2013 Dec 17;129(3):400–412. doi: 10.1111/jnc.12617

Fig. 3. Incorporation of glutamate radioactivity into lipid classes upon hypoxia in neuronal cell line.

Fig. 3

SH-SY5Y cells were preconditioned in serum-free MEM medium under normoxic (19% O2) or hypoxic (1% O2) conditions for 24 h, and for another 18 h with 2 µCi of [U-14C]glutamate. Lipids were extracted from cells after 42 h of incubation using chloroform/methanol by the Folch protocol, separated by TLC and radioactivity of individual lipid classes was analyzed by liquid scintillation counting. Abbreviations are: CE, cholesteryl esters; TG, TAGs; FFA, free fatty acids; DAG, diacylglycerols; Ch, cholesterol; PL, phospholipids; * - significantly different as compared to 19% O2. Values are mean ± SD, n = 3.