Table 1.
Shoot and root phenotypes of 35-day-old wild type (‘Frisson’, 212+ ) and various single- and double-mutant combinations of brassinosteroid-deficient lk and AON mutants Psnark, Psclv2 and Psrdn1 (LE/le genotype indicated in parentheses)
| Genotype | Leaves expanded | L1–6 | Shoot d. wt | Root d. wt | Individual nodule d. wt |
|---|---|---|---|---|---|
| ‘Frisson’ (le) | 8·25 ± 0·31a | 62·3 ± 3·6a | 188·2 ± 8·8a | 52·6 ± 2·9a | 0·18 ± 0·06ab |
| Psnark (le) | 8·58 ± 0·27a | 42·8 ± 2·1b | 67·9 ± 10·4b | 25·0 ± 2·3b | 0·06 ± 0·01a |
| Psclv2 (le) | 8·08 ± 0·15a | 68·0 ± 4·2a | 117·9 ± 5·4c | 32·8 ± 2·8b | 0·04 ± 0·01a |
| Psrdn1 (le) | 7·08 ± 0·33a | 52·7 ± 0·8a | 121·9 ± 10·4c | 38·1 ± 2·8ab | 0·03 ± 0·01a |
| 212+ (LE) | 8·75 ± 0·21a | 184·5 ± 3·8c | 266·9 ± 7·5d | 92·5 ± 5·9c | 0·17 ± 0·03ab |
| lk (LE) | 5·83 ± 0·17b | 38·5 ± 2·4b | 66·8 ± 6·2b | 23·9 ± 3·9b | 0·11 ± 0·03ab |
| Psnark lk (LE) | 7·75 ± 1·2a | 38·2 ± 6·3b | 85·4 ± 9·9bc | 26·68 ± 4·2b | 0·07 ± 0·01a |
| Psclv2 lk (LE) | 7·92 ± 0·93a | 39·7 ± 7·4b | 60·37 ± 8·4bc | 18·9 ± 1·1b | 0·23 ± 0·05ab |
| Psrdn1 lk (LE) | 7·83 ± 0·77a | 39·3 ± 2·2b | 87·8 ± 7·9b | 24·0 ± 2·6b | 0·13 ± 0·02ab |
The number of leaves expanded, length of shoot from node 1 to 6 (L1–6) and shoot, root and individual nodule dry weight (mg) are indicated.
Values within a column with different letters indicate significant differences (ANOVA, P < 0·01).
Values are the mean ± s.e. (n = 5–6).