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Primers for nested methylation-specific polymerase chain reaction.
| Primer names | Sequences, 5′-3′ | Size, bp |
|---|---|---|
| Nested primer | 44 | |
| Forward | AGATAAGGTTTAGGGAAGGYGTT | |
| Reverse | AACAACCRCAATAACCAATCTACT | |
| Methylated p33ING1b primer | 151 | |
| Forward | CGGATGGCGTAGGCGCGGGAGTC | |
| Reverse | CCGAACACGAACGAAAATAACGACGC | |
| Unmethylated p33ING1b primer | 151 | |
| Forward | TGGATGGTGTAGGTGTGGGAGTT | |
| Reverse | CCAAACACAAACAAAAATAACAACACA |
p33ING1b, p33 inhibitor of growth 1b.
