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Expression of MMP1 and CK19 mRNA in northern blot analysis. EGF increased the expression of MMP1. By contrast, the expression of CK19 mRNA was evidently reduced by EGF, even at the lowest concentration of 10 ng/ml. GAPDH mRNA was used as an internal marker of applied RNA amounts. EGF, epidermal growth factor; MMP1, matrix metalloproteinase 1; CK19, cytokeratin 19; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
