. 2014 Feb 20;7(5):1618–1624. doi: 10.3892/ol.2014.1897

Table I.

Conventional qPCR analyses of CXCL12, transcript variants 1–4; CXCR4, transcript variants 1–2 and CXCR7.

cDNA	GenBank accession number	Primer	Primer sequence (5′-3′)	Position	PCR product size (bp)
CXCL12, transcript variant 1	NM_199168	S	TACAGATGCCCATGCCGATT	174–193	262
CXCL12, transcript variant 1		A	GCCCTTTCATCTCTCACAAGGT	414–435
CXCL12, transcript variant 2	NM_000609	S	TGTGCATTGACCCGAAGCTA	301–320	144
CXCL12, transcript variant 2		A	CAGGCCCTTCCCTAACACT	426–444
CXCL12, transcript variant 3	NM_001033886	S	ACTGTGCCCTTCAGATTGTAGCC	253–275	260
CXCL12, transcript variant 3		A	AGCAAATTTACAAAGCGCCGAGA	490–512
CXCL12, transcript variant 4	NM_001178134	S	TACAGATGCCCATGCCGATT	174–193	196
CXCL12, transcript variant 4		A	CGCTGATCAGGTTGTTTAAAG	349–369
CXCR4, transcript variant 1	NM_001008540	S	CTACATTAATTCTCTTGTGCC	175–195	241
CXCR4, transcript variant 1		A	ATTTTCTTCACGGAAACAGG	396–415
CXCR4, transcript variant 2	NM_003467	S	CTGAGTGCTCCAGTAGCC	61–68	281
CXCR4, transcript variant 2		A	TGCAGCCTGTACTTGTCC	314–331
CXCR7	NM_020311.2	S	CAAAGCTGCCATCTAGAGG	16–34	252
CXCR7		A	CTGATGTCCGAGAAGTTCC	249–267
MRLP19 (reference gene)	NM_014763	S	TCCTCGGGTCCAGGAGATT	529–547	58
MRLP19 (reference gene)		A	CAAGCTATCATCCAGCCGTTT	566–586

Oligonucleotide sequences for S and A primers. MRPL19 was the reference gene. S, sense; A, antisense; MRPL19, mitochondrial ribosomal protein L19; qPCR; quantitative polymerase chain reaction.