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Relative mRNA expression of miR-34a on putative target genes in (A) 786-O and (B) Caki-1 cells transfected with miR-34a mimics or the negative control. Data are expressed as the means ± SD. *P<0.05 and **P<0.01, compared with the negative control. All the experiments were performed three times independently. miR-34a, microRNA-34a; SIRT1, silent information regulator 1; CDK6, cyclin-dependent kinase 6; E2F3, E2F transcription factor 3; CCNE2, cyclin E2.
