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. 2014 Mar 4;7(5):1689–1694. doi: 10.3892/ol.2014.1931

Figure 4.

Figure 4

Downregulation of Notch1 by miR-34a in the RCC cell lines. (A) Quantitative polymerase chain reaction analysis of Notch1 mRNA expression level and (B) western blot analysis of Notch1 protein expression level in the RCC cell lines 48 h after transfection with miR-34a mimics compared with the negative control. Data are expressed as the mean ± SD. *P<0.05 and **P<0.001, compared with the negative control. All the experiments were performed three times independently. miR-34a, microRNA-34a; RCC, renal cell carcinoma; GAPDH, glyceraldehyde-3-phosphate-dehydrogenase.