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Glycosaminoglycan (GAG)/DNA ratio relative to directly harvesting at day 0 (D0) in unloaded constructs (F2, F4) after loading for 2 or 4 weeks (L2, L4) or free swelling for 2 weeks followed by loading for 2 weeks (F2L2) (P > 0.05). Data from three experiments each run in triplicates are shown
