Table 1.

Sequences of oligonucleotide primers and probes used for real-time polymerase chain reaction (PCR)

Gene (bovine)	Symbol	Forward primer (5′–3′)	Reverse primer (5′–3′)	Probe (5′FAM/3′TAMRA)
Collagen 1A2	COL1A2	TGC AGT AAC TTC GTG CCT AGC A	CGC GTG GTC CTC TAT CTC CA	CAT GCC AAT CCT TAC AAG AGGRECAN CAA CTG C
Collagen 2A1	COL2A1	AAG AAA CAC ATC TGG TTT GGA GAA A	TGG GAG CCA GGT TGT CAT C	CAA CGG TGG CTT CCA CTT CAG CTA TGG
Aggrecan	ACAN	CCA ACG AAA CCT ATG ACG TGT ACT	GCA CTC GTT GGC TGC CTC	ATG TTG CAT AGA AGA CCT CGC CCT CCA T
Cartilage oligomeric matrix protein	COMP	CCA GAA GAA CGA CGACCA GAA	TCT GAT CTG AGT TGG GCA CCT T	ACG GCG ACC GGA TCC GCA A
Proteoglycan-4 (SZP)	PRG-4	GAG CAG ACC TGA ATC CGT GTA TT	GGT GGG TTC CTG TTT GTA AGT GTA	CTG AAC GCT GCC ACC TCT CTT GAA A