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. 2014 Feb 7;239(5):1041–1053. doi: 10.1007/s00425-014-2034-1

Table 1.

Primers for DNA in situ probes synthesis

Oligo name Sequencea Product size (bp)
IRX10-AS-F TCCTCCAATTGGCCTTACTG 730 Antisense probe
IRX10-AS-T7 GAATTGTAATACGACTCACTATAGGGGTGGAAAGCATCACCTGGTTa
IRx10-SP-T7 GAATTGTAATACGACTCACTATAGGGGAAGGACCCCAGATGTCTCA 730 Sense probe
IRX10-SP-R GTGGAAAGCATCACCTGGTT
IRX9-AS-F GGTGCTTAGAAAGACAGGCATTAT 474 Antisense probe
IRX9-AS-T7 AATTGTAATACGACTCACTATAGGGTCATCCTCAAGAGCTACTTGTTTG
IRX9-SP-T7 GAATTGTAATACGACTCACTATAGGGGGTGCTTAGAAAGACAGGCATTAT 474 Sense probe
IRX9-SP-R TCATCCTCAAGAGCTACTTGTTTG
Gux-AS2-F TGCTCATGAAAAGTGGTGGA 301 Antisense probe
Gux-AS-T7 GAATTGTAATACGACTCACTATAGGGCACAGGCCAGAAAGAGATGC
Gux-SP-F7 GAATTGTAATACGACTCACTATAGGGTGCTCATGAAAAGTGGTGGA 301 Sense probe
Gux-SP-R CACAGGCCAGAAAGAGATGC

aUnderlined sequence for T7 transcription site