Protein nitrosylation is increased in atherosclerotic ApoE−/− mouse thoracic aortae. (a, b) Representative cross-sectional images of nitrotyrosine stained C57/BL-6 and 4 month hfd ApoE−/− mouse thoracic aortae (200× magnification). (c, d) Smooth muscle actin staining. (e) Mean DAB staining/nuclear area (%) for nitrotyrosine and actin. (f) H&E stained aorta from 4 month hfd ApoE−/−. L = lumen, SM = smooth muscle, P = plaque, FC = fibrous cap, +ve = areas of positive staining ***p < 0.001 vs. C57/BL-6, n = 5 mice per group.