Expression levels of SERCA2b are progressively reduced while PMCA levels are increased in aortic smooth muscle from ApoE−/− mice. (a, b) Representative immunoblots and averaged protein expression data for SERCA2b, pan-PMCA and GAPDH. Protein loads were 2.5, 5, 7.5 μg for SERCA and 10, 15, 20 μg for PMCA. Slopes of plotted band densities were standardised to GAPDH. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. C57/BL-6, minimum of 5 independent experiments.