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. 2014 Apr 16;5:3651. doi: 10.1038/ncomms4651

Figure 2. Phenotypes of CO transgenic complementation lines.

Figure 2

(a) Diagram of the inserts for transgenic complementation lines that have a 3X or 4X CO promoter fused to CO-coding region: the promoter (grey block), the tandem repeat (inverted triangle), UTRs (green blocks), exons (black block) and an intron (thin line). (b–c) Cumulative flowering time of unvernalized (b) and vernalized (c) complementation lines of CO 3X (n=358 unvernalized plants, n=298 vernalized plants) and CO 4X (n=414 unvernalized plants, n=360 vernalized plants). Black diamonds indicate CO 4X fri-(Col-0), grey squares CO 3X fri-(Col-0), empty diamonds CO 4X FRI-Sf2 and empty squares CO 3X FRI-Sf2. (d–g) Mean phenotypes in the FRI-Sf2 and fri− backgrounds are shown in unvernalized (d,f) and vernalized (21 days at 4 °C) plants (e,g). The dark tick marks indicate the s.e. Post hoc Tukey’s test in REML mixed model ***P<0.0005, **P<0.005, *P<0.05. NS, not significant. Error bars indicate s.e.m.