Fig. 3.

a–l Genomic in situ hybridization of the putative alien chromosomes of G. australe in the G. hirsutum background shows the chromosomal constitutions. a Mitotic chromosome spread of the 52 chromosomes of G. hirsutum. b Mitotic chromosome spread of the 26 chromosomes of G. australe. c, d and e Mitotic chromosome spread showing the 52 G. hirsutum (blue) chromosomes and individual chromosomes 3G^a, 6G^a, and 10G^a of G. australe (red, white arrowhead), respectively. f, g and h Mitotic chromosome spread showing the 52 G. hirsutum (blue) chromosomes and two (2G^a, 10G^a), two (10G^a, 13G^a) and three (7G^a, 8G^a, 10G^a) chromosomes of G. australe (red, white arrowhead), respectively. i Meiotic chromosome spread showing 26 bivalents of G. hirsutum. j, k and l Meiotic chromosome spread showing 26 bivalents of G. hirsutum plus 1 univalent of chromosomes 5G^a, 6G^a, and 10G^a from G. australe (white arrowhead), respectively. Scale bar 3 μm