Fig. 7.

a–e G. australe-specific DNA fragments detected using SSR markers during backcrossing were deleted in some MAALs in G. hirsutum. a–e Deleted G. australe-specific amplicons were detected by individual chromosome-specific SSR primers for NAU6728 (2G^a), NAU5486 (5G^a), MUSS266 (10G^a), BNL193 (13G^a), and CIR414 (11G^a), respectively. P1 G. hirsutum, P2 G. australe. F ₁ the hexaploid of G. hirsutum and G. australe. N indicates MAAL-2G^a (a), MAAL-5G^a (b), MAAL-10G^a (c), MAAL-13G^a (d), and MAAL-11G^a (e). M is the molecular size marker (50 bp ladder). Arrows indicate the deleted G. australe chromosome-specific markers