Table 1.
SSR primers used to screen for G. australe chromosomes in the alien addition lines
| Chr | 1G | 2G | 3G | 4G | 5G | 6G | 7G | 8G | 9G | 10G | 11G | 12G | 13G |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NAU458-230 | NAU3733-490 | shin-250 | NAU6992-200 | NAU3095-210 | BNL3359-200 | NAU5325-180 | BNL2597-220 | BNL686-150 | MUSS266-180 | NAU5461-210 | NAU3032-390 | NAU3685-120 | |
| NAU3346-280 | JESPR-156-90 | TMK08-190 | BNL4030-130 | NAU4907-350 | BNL2569-680 | BNL1694-240 | NAU3010-160 | NAU2739-190 | STV031-160 | dPL0522-195 | NAU3862-270 | BNL193-100 | |
| NAU3714-170 | STV030-135 | BNL3955-150 | JESPR-65-130 | NAU2232-450 | BNL3103-190 | BNL1604-110 | NAU5130-330 | NAU6701-390 | NAU2549-260 | BNL1034-200 | BNL3537-170 | NAU7471-160 | |
| NAU3056-630 | NAU4009-160 | NAU5333-150 | dPL0390-230 | JESPR236-400 | NAU2397-240 | TMB09-280 | dPL0862-220 | NAU6517-300 | NAU6720-150 | Gh508-200 | NAU4914-200 | dPL0286-220 | |
| NAU5172-180 | NAU2633-240 | JESPR101-160 | NAU3508-250 | BNL3535-145 | dc40121-180 | cot030-200 | shin0384-200 | NAU6751-135 | NAU4973-170 | Gh83-140 | NAU1558-380 | cgr5390-340 | |
| MUCS322-450 | NAU7342-270 | NAU1028-120 | MUCS271-360 | dPL0137-140 | BNL3806-160 | NAU2620-320 | cgr5503-120 | NAU5508-160 | Gh424-95 | NAU5436-370 | BNL341-160 | BNL1079-200 | |
| BNL2646-120 | Gh51-130 d | NAU6129-280 | BNL358-250 | BNL390-210 | Gh537-160 | NAU4956-280 | Gh573-145 | NAU2709-200 | TMF09-240 | BNL2632-340 | NAU7594-500 | BNL3280-200 | |
| NAU4073-180 | NAU6692-270 | JESPR-304-140 | cgr5566-155 | NAU6406-230 | JESPR229-115 | cgr5149-120 | cgr5202-150 | JESPR208-160 | NAU0922-220 | cgr5233-430 | cgr5787-120 | NAU3203-160 | |
| NAU2814-230 | NAU4022-200 | NAU2859-270 | NAU3437-250 | NAU5486-270 | dPL0702-210 | NAU2597-190 | NAU3904-240 | JESPR110-170 | NAU1280-130 | Gh434-150 | NAU3442-320 | BNL2652-280 | |
| cgr5309-140 | NAU3573-800 | NAU2898-230 | NAU7290-200 | Gh109-120 | NAU2641-180 | cgr5181-120 | JESPR291-170 | NAU3414-240 | NAU1169-300 | cgr5148-140 | NAU1119-220 | NAU6724-370 | |
| dc40046-240 | JESPR-220-150 | cgr5117-130 | MUSS519-160 | cgr6572-330 | STV031-370 | CIR414-160 | BNL2578-250 | dPL0864-200 | |||||
| NAU6728-330 | Gh229-130 | NAU3502-240 | NAU3414-250 | NAU2602-310 | NAU4925-600 | BNL2571-360 | |||||||
| cgr5510-150 | NAU864-170 | Gh132-170 | NAU3271-200 | NAU3861-450 | |||||||||
| cgr5803-120 | NAU486-230 | NAU3843-280 | |||||||||||
| cgr6240-240 | NAU429-220 | ||||||||||||
| Gh182-190 | NAU6658-180 | ||||||||||||
| JESPR122-320 | |||||||||||||
| NAU3631-270 | |||||||||||||
| Total | 10 | 12 | 10 | 11 | 18 | 12 | 10 | 10 | 13 | 11 | 16 | 13 | 14 |
| Position | 0.0–107.3 | 5.6–110.4 | 14.0–119.5 | 3.5–105.9 | 10.9–185.4 | 8.5–123.9 | 2.4–121.1 | 0.0–149.9 | 0.0–143.9 | 0–112.4 | 5.8–157.7 | 15.3–126.2 | 8.8–103.1 |
| Mean densitya | 10.7 | 8.7 | 10.6 | 9.3 | 9.7 | 9.6 | 11.9 | 15 | 11.1 | 10.2 | 9.5 | 8.5 | 6.7 |
| GDC (cM)b | 107.3 | 104.8 | 105.5 | 102.4 | 174.5 | 115.4 | 118.7 | 149.9 | 143.9 | 112.4 | 151.9 | 110.9 | 94.3 |
| PCC (%)c | 85.3 | 93.5 | 83.6 | 80.2 | 91.9 | 83.7 | 91.7 | 90.3 | 95.2 | 97.3 | 83.9 | 83.0 | 80.5 |
a Genetic distance (cM) between two adjacent markers on a chromosome
b GDC genetic distance coverage (cM)
c PCC percentage of chromosome covered by markers (%)
d Boldface type indicates the molecular markers that are deleted in the MAALs