Table 1.
Chronobiological analysis of liver GABA-T parameters: mRNA, protein amount, and activity.
| GABA-T mRNA | GABA-T protein | GABA-T activity | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Hg | Mt | Hg | Mt | |||||||
| AL | DRF | AL | DRF | AL | DRF | AL | DRF | AL | DRF | |
| Rhythm (%) | — | — | — | — | — | — | — | 54.8 | 40.3 | 38.4 |
| Mesor | 0.9 | 0.8 | 1.1 | 1.5 | 0.7 | 0.9 | 5.2 | 5.4 | 2.4 | 2.0 |
| Amplitude | 0.3 | — | — | — | — | — | — | 1.55 | 0.48 | 0.50 |
| Acrophase (h:min) | 7:40/15:15/22:50 | — | — | — | — | — | — | 9:56 | 5:56 | 4:14 |
| Period | 8 h | — | — | — | — | — | — | 24 h | 24 h | 24 h |
Chronos-Fit analysis was performed to evaluate rhythmicity for GABA-T parameters in liver. Acrophases in GABA-T mRNA from AL group were repeated each 7 h with 40 min. Hg: liver homogenate, Mt: mitochondrial fraction, AL: ad libitum, and DRF: daytime restricted feeding. (—) means that no rhythmic pattern was detected.