Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 21;11:26. doi: 10.1186/1742-4690-11-26

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Du et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

EIAV replication kinetics and temperature sensitivity assays. (A) Replication analysis of wild-type (WT) EIAV and EIAV with mutations at position d. The procedure employs ED cells which are infected by the virus in sets of three. EIAV replication kinetics is assayed using qPCR, by measuring the virions in the supernatant. (B) Temperature sensitivity measurements; at 41°C, each virus experiences a infection decrease compared to a temperature of 37°C. The same behavior is observed for WT but to a different degree. (C) The infection ratios from 37 to 41°C. The V505T mutation exhibits significant sensitivity with a considerable drop in infection as the temperature is increased. The L512T mutant is also affected in a similar manner, while the other mutants tested show minimal differences. Results are an average of three independent experiments.