Table 1.
Primers used to perform qPCR of selected candidates
| All conditions | |||
|---|---|---|---|
|
miRNA |
Sequence |
Forward primer |
Reverse primer |
|
mir-2-5p |
UAUCACAGCCAGCUUUGAUGUGC |
TCACAGCCAGCTTTGATG |
GTCCAGTTTTTTTTTTTTTTTGCAC |
|
mir-46-3p |
UGUCAUGGAGUCGCUCUCUUCA |
TGTCATGGAGTCGCTCTC |
GGTCCAGTTTTTTTTTTTTTTTGAAGA |
|
mir-47-3p |
UGUCAUGGAGGCGCUCUCUUCA |
CATGGAGGCGCTCTC |
GGTCCAGTTTTTTTTTTTTTTTGAAGA |
|
UV | |||
|
mir-357-3p |
AAAUGCCAGUCGUUGCAGGAGU |
TGCCAGTCGTTGCAG |
GGTCCAGTTTTTTTTTTTTTTTACTC |
|
miR-57-5p |
UACCCUGUAGAUCGAGCUGUGUGU |
AGTACCCGTATAAGTTTCTGCT |
GGTCCAGTTTTTTTTTTTTTTTCTC |
|
miR-55-3p |
UACCCGUAUAAGUUUCUGCUGAG |
GCAGTATGACACTGAAGCGT |
GTCCAGTTTTTTTTTTTTTTTCGGT |
|
Heat-stress | |||
|
miRNA |
Sequence |
Forward primer |
Reverse primer |
|
miR-64-5p |
UAUGACACUGAAGCGUUACCGAA |
GCAGTATGACACTGAAGCGT |
GTCCAGTTTTTTTTTTTTTTTCGGT |
|
miR-86-5p |
UAAGUGAAUGCUUUGCCACAGUC |
AGTAAGTGAATGCTTTGCCA |
GTCCAGTTTTTTTTTTTTTTTGAC |
|
miR-794-5p |
UGAGGUAAUCAUCGUUGUCACU |
CAGTGAGGTAATCATCGTTGT |
GTCCAGTTTTTTTTTTTTTTTAGTGA |
|
Hypoxia N2 + hif-1 | |||
|
miRNA |
Sequence |
Forward primer |
Reverse primer |
|
miR-230-3p |
GUAUUAGUUGUGCGACCAGGAGA |
GGTATTAGTTGTGCGACCAG |
GGTCCAGTTTTTTTTTTTTTTTCTC |
|
miR-357-3p |
AAAUGCCAGUCGUUGCAGGAGU |
TGCCAGTCGTTGCAG |
GGTCCAGTTTTTTTTTTTTTTTACTC |
|
miR-58-3p |
UGAGAUCGUUCAGUACGGCAAU |
CAGTGAGATCGTTCAGTACG |
GGTCCAGTTTTTTTTTTTTTTTATTGC |
|
Hypoxia N2 | |||
|
miRNA |
Sequence |
Forward primer |
Reverse primer |
|
miR-52-5p |
CACCCGUACAUAUGUUUCCGUGCU |
ACCCGTACATATGTTTCCGT |
GGTCCAGTTTTTTTTTTTTTTTAGCA |
|
miR-254 |
UGCAAAUCUUUCGCGACUGUAGG |
GCAAATCTTTCGCGACTGT |
GGTCCAGTTTTTTTTTTTTTTTCCT |
| miR-241-3p | AUUGUCUCUCAGCUGCUUCAUC | AGATTGTCTCTCAGCTGCT | GTCCAGTTTTTTTTTTTTTTTGATGAAG |