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. 2014 Mar 22;9:80. doi: 10.1186/1748-717X-9-80

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Copyright © 2014 Large et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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NAC treatment abolishes the non-linear characteristics of γH2AX induction in EA.hy926 EC following low-dose X-irradiation. EA.hy926 EC were stimulated with TNF-α (20 ng/ml) or mock-treated and were irradiated in the presence of NAC (10 mM, applied 4 h before irradiation). At 24 h after irradiation relative DCF-fluorescence (A) and γH2AX foci induction (B) were analysed as described before. Data represent means ± SD from at least three independent experiments. *p < 0.05 vs. 0.3 Gy and 0.7 Gy irradiated EC. **p < 0.01 vs. relative DCF controls.