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(A) Structures of 1–7. (B) Site-specific incorporation of 1–7 into sfGFP at its S2 site. N/A indicates no NCAA was available in the medium. ND represents nondetected. (C) Deconvoluted ESI-MS of sfGFP with NCAAs 1–7 incorporated at the S2 position. sfGFP-X in which X is one of 1–7 represents the specific sfGFP variant with X incorporated at its S2 site.
