Table 3.

Quantitation of ARS staining of calcium deposits and ALP enzyme.

	EX(−)	EX(+)	OG(−)	OG(+)
ARS day 7	0.12 ± 0.01	0.11 ± 0.01	0.16 ± 0.02	0.20 ± 0.05
EX versus OG			P = 2.15E − 05	P = 0.002
(−) versus (+)				P = 0.037
ARS day 14	0.24 ± 0.06	0.20 ± 0.02	0.31 ± 0.06	0.92 ± 0.19
EX versus OG			P = 0.007	P  =  6.56E − 10
(−) versus (+)				P = 2.02E − 9
ALP day 14	0.09 ± 0.01	0.09 ± 0.01	0.12 ± 0.02	0.18 ± 0.04
EX versus OG				P = 0.0004
(−) versus (+)				P = 0.005

The ARS staining of calcium deposits and measurements of an OD of 570 nm after cetylpyridinium chloride elution were performed at days 7 and 14. Quantitation of calcium deposits revealed a highly significant increase of an OD of 570 nm values in osteogenic differentiated cells (OG) compared to the corresponding undifferentiated controls (EX). Mechanical stimulation revealed a significant increase in calcium deposits in the OG(−) cells compared to the OG(+) cells. Quantitation of ALP enzyme at day 14 revealed a significant increase in osteogenic differentiated cells (OG) compared to the corresponding undifferentiated controls (EX). The values are mean ± SD of independent experiments performed in triplicate (n = 12); *P < 0.05.