Table 4.
Sequences and fluorescent labels of primers for six microsatellites of Robinia pseudoacacia
| Locus | Fluorescence label | Motif | Primer sequence | Size range (bp) | T m (°C) | GenBank accession no. |
|---|---|---|---|---|---|---|
| Rops08 |
HEX |
(CA)8TA(CA)3 |
TTCTGAGGAAGGGTTCCGTGG |
192–212 |
63 to 52 |
AB075033 |
| GTTAAAGCAACAGGCACATGG | ||||||
| Rp206 |
HEX |
(GT)9 |
GCCAAATCCCATTAGATCACAGTTGA |
200–232 |
65 to 58 |
AB353932 |
| AGAAGTTAGACTTACGTGCTGC | ||||||
| Rops05 |
FAM |
(AC)2GC(AC)7 |
TGGTGATTAAGTCGCAAG |
114–148 |
56 |
AB075031 |
| GTGGTTGTGACTTGTACGTAAGTC | ||||||
| Rops06 |
FAM |
(GT)3ACA(GT)11 |
CTAAGGAGGTGCTGACCCTC |
114–146 |
65 to 58 |
AB075032 |
| TTAATCTGTGATGGGACACTG | ||||||
| Rp109 |
TAMRA |
(AG)17 |
GAGGAATCACAAAACCGTTTGG |
119–151 |
65 to 63 |
AB353930 |
| TGGGATTTGAGAGAGTGGTGGTG | ||||||
| Rp200 | TAMRA | (AG)23 | GGTTTCTTTGTTCACCTGCTCTGG |
160–185 | 65 to 60 | AB353933 |
| ACCTACGTGTCCACGGCTCT |