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. 2014 Apr 24;4:4777. doi: 10.1038/srep04777

Figure 3. Stimulation with AP-PG also induces viperin mRNA after treatment with cycloheximide.

Figure 3

(A), (B) RAW264.7 cells were stimulated for the indicated times with 100 μg/ml of AP-PG or poly (I:C), and then the total RNAs isolated from the cells were subjected to real-time RT-PCR analysis using specific primer sets. The data represent relative expression values compared with the mRNA expression at the initial time point after normalization with the GAPDH mRNA expression. (C) RAW264.7 cells were treated with 5 mM cycloheximide (CHX) for 30 min. Subsequently, the cells were stimulated with or without 100 μg/ml of AP-PG. After the indicated incubation periods, the cells were harvested, and the viperin mRNA expression was analyzed by real-time RT-PCR. The data are represented as relative expression values compared with the mRNA expression in untreated cells after the normalization with GAPDH mRNA expression. Error bars indicate standard deviations calculated by three independent experiments.