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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Glia. 2013 Aug 6;61(10):1595–1606. doi: 10.1002/glia.22523

Figure 1.

Figure 1

CNPase knockout confirmation and cell type identification. The complete lack of both type 1 (CNP1) and type 2 (CNP2) isoforms of CNPase was confirmed by Western blot on protein extracts from CNPase +/+ and -/- mouse cortical tissue using an anti-CNPase antibody. GAPDH is shown as a loading control (A). A neuron specific beta III-tubulin antibody and a FITC-conjugated secondary antibody was used to confirm pure cultures of primary mouse neurons (Green, DAPI, blue; Scale bar = 50 microns) (B). An anti-CNPase antibody was used to detect mature mouse oligodendrocyte cultures (Green, DAPI, blue; Scale bar = 50 microns) (C).