Figure 29.

Staining of neurogenesis markers in the white matter of the parahippocampal gyrus. Boxes with numbers 8 and 9 in Figure 26 indicate the locations of lines 1 and 2, respectively, in this panel. Figures (A–C) show the boundary between the subicular complex and the white matter, which contains fibers that run from the subicular complex toward the fimbria through the SVZ of the hippocampus proper. DCX (green) stains fibers in the inferior-superior direction (upper arrowhead in (B)), transversally cut fibers (lower arrowhead in (B)) in the subicular complex and fibers running in an anterior-posterior direction (arrow in (B)) in the white matter. DCX staining in the white matter (E) depicts a pattern of DCX-positive fibers intercalated with rows of oligodendrocyte nuclei (arrows in (E) and (H)). (A) and (D), GFAP staining (red); (C) and (F), βIII-tubulin staining. Figures (G) and (H) are negative control images acquired from the counterpart locations in another section. Scale bars: (A), (D), (G) = 50 μm; (B), (C), (E), (F), (H) = 100 μm.