Table 1.
Primary antibodies
| Antibody | Type | Manufacturer | Catalog no. | Dilution | Phenotype |
|---|---|---|---|---|---|
| Nestin |
Rb |
Millipore* |
AB5922 |
1:200 (F), 1:5000 (C) |
NSC |
| Nestin |
Rb |
Abcam** |
ab93666 |
1:100 |
NSC |
| Nestin |
Ms |
Abcam |
ab22035 |
1:100 |
NSC |
| CD133 |
Rb |
Abcam |
ab66141 |
1:100 |
NSC |
| GFAP |
Ms |
Abcam |
ab80842 |
1:1200 |
NSC, astrocyte |
| Vimentin |
Ms |
Dako*** |
M725 |
1:500 |
NSC, astrocyte |
| PCNA |
Ms |
Dako |
M879 |
1:1000 |
Cell cycle |
| Ki-67 |
Ms |
Dako |
M7187 |
1:500 |
Cell cycle |
| Glut-1 |
Ms |
Abcam |
ab40084 |
1:100 |
Blood-CSF barrier |
| DCX |
Rb |
Abcam |
ab18723 |
1:250 |
Neuroblast, immature neuron |
| βIII-tubulin |
Ms |
Abcam |
ab47967 |
1:100 |
Immature and mature neuron |
| MAP-2 | Rb | Abcam | ab75059 | 1:300 | Mature neuron |
CSF: cerebrospinal fluid; GFAP: glial fibrillary acid protein; MAP-2: microtubule-associated protein 2; Ms: mouse; NSC: neural stem cell; PCNA: proliferating cell nuclear antigen; Rb: rabbit. *Billerica, MA, USA; **Cambridge, MA, USA; ***Dako Denmark, Glostrup, Denmark. (C): chromogenic immunohistochemistry in Figures 2, 3, 4, 5, 6, and 7; (F): fluorescence immunohistochemistry in Figure 2.