Skip to main content
. Author manuscript; available in PMC: 2015 Apr 25.
Published in final edited form as: Neuroscience. 2014 Feb 12;266:80–90. doi: 10.1016/j.neuroscience.2014.01.066

Fig. 5. Homer labeling at postsynaptic density (PSD).

Fig. 5

Pre-embedding immunogold labeling of perfusion-fixed brain (A, B, C) and dissociated hippocampal cultures (D, E, F) show that four different antibodies distinguishing Homer isoforms are all concentrated at the PSD. Asymmetric synapses were sampled from different regions of the perfusion-fixed brain: (A) Homer 1 labeling from hippocampus, (B) Homer 2 labeling from cerebral cortex, (C) Homer 3 labeling of Purkinje spines in the outer molecular layer of cerebellum. Asymmetric synapses sampled from dissociated hippocampal cultures also show concentrated labeling at the PSD for Homer 1b/c (D), Homer 2 (E) and Homer 3 (F). Bracket in B marks the depth (~30 nm) of the inner layer of dense material at the PSD. Virtually no labeling was detected at the peri-PSD locations (parenthesis in E), or around the periphery of PSD (arrows in F). Scale bar = 0.1 μm.