Figure 1.

Aluminum worsens 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. (a) C57BL6 mice (n=14 per group) were fed with aluminum citrate (AluCi) or aluminum phosphate (AluP) (1.5 mg of Al element kg⁻¹ per day) or with phosphate buffer saline (PBS) for 31 days. At day 28, colitis was induced by intrarectal administration of TNBS. Four days after colitis induction, mice were euthanized and colitis parameters were assessed. (b) The macroscopic Wallace score was determined as described in Methods. (c, d) Histopathological changes in colon tissues were examined by May-Grünwald and Giemsa (MGG) staining and scoring was performed as described in Methods. (e) Myeloperoxidase (MPO) activity was measured in colonic lesions. Results are expressed as the percentage of variation compared with TNBS-treated mice.