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. Author manuscript; available in PMC: 2014 Aug 24.
Published in final edited form as: Dev Cell. 2014 Feb 24;28(4):459–473. doi: 10.1016/j.devcel.2014.01.020

Figure 3. Genes and complexes required for GSC maintenance.

Figure 3

(A–D) Identified protein complexes required for GSC maintenance (Figure S3, Table S2). Complexes are enlarged from Figure 2E. RNAi against one representative gene from each complex is shown and other genes in the complex have a similar phenotype when knocked down by RNAi. (A) Knockdown of Uba2 or Aos1 by two independent RNAi constructs using MTD-Gal4 or UAS-dcr2; nanos-Gal4 results in a depletion of the germline. MAT-Gal4 mediated knockdown of these genes does not induce an obvious phenotype at later stages of oogenesis. (B-D) Knockdown of members of the respective complex members results in a depletion of the germline. Independent shRNA constructs are shown in inserts.

(E) Knockdown of igru (CG11266) with MTD-Gal4 results in a depletion of the germline.

(F) Trap-mediated loss-of-function validates the loss of vasa-positive germline cells upon loss of igru function.

(G) igru::GFP is expressed throughout the germline including high level expression in GSCs and low expression levels in the cystocyte region (top panel). Remaining germline cells stain positive for Vasa and cleaved Caspase (lower panel).

Scale bars: 20 μm.