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. Author manuscript; available in PMC: 2014 Apr 24.
Published in final edited form as: Neuroscience. 2012 Jul 13;222:181–190. doi: 10.1016/j.neuroscience.2012.07.010

Figure 1.

Figure 1

Met-enkepahlin (Met-Enk) inhibits solitary tract evoked TH-EGFP synaptic responses in horizontal brainstem slices. A. Visualization of the NTS horizontal brain-slice preparation from a TH-EGFP mouse using DIC and florescence. Scale bar = 0.5 mM. B. Representative traces ST-stimulated EPSCs (ST-EPSCs), with 10 overlapping sweeps shown. Met-Enk significantly inhibited the amplitude of the ST-EPSCs. This effect was reversed following a 5–10 minute wash. C. A graph showing the effect of Met-Enk on ST-EPSC amplitude over time. Met-Enk rapidly decreased the ST-EPSC amplitude in a manner that was quickly reversed following wash (n=5). D. A graph showing Met-Enk increases the paired pulse ration (PPR, EPSC2/EPSC1) (n=7). E. A graph showing Met-Enk increases the failure rate (% failures = (number of failures / total stimulations) * 100) in TH-EGFP neurons (n=7). * p<0.05 (One way ANOVA).