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. Author manuscript; available in PMC: 2014 Apr 24.
Published in final edited form as: Cold Spring Harb Protoc. 2010 Dec 1;2010(12):pdb.prot5542. doi: 10.1101/pdb.prot5542

MOVIE 2.

MOVIE 2

Disruption of the fate of the C blastomere using pal-1(RNAi) knockdown results in delayed and minimal con- tact between the C and the ABar blastomeres before division of ABar, causing the ABar spindle to be misaligned. (Left) Contact is minimal and is delayed between the C blastomere (membrane near contact outlined in blue) and the ABar blastomere (membrane near contact outlined in green). The focal depth (14 μm) of greatest contact is shown. (Right) In lieu of a polarizing signal from the C blastomere, the ABar blastomere (right line) adopts the default spindle orien- tation and divides parallel with the ABpr blastomere (left line). The focal depth is 7 μm. (To view movie, see doi: 10.1101/pdb.prot5542 online at www.cshprotocols.org.)