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. Author manuscript; available in PMC: 2014 Apr 24.
Published in final edited form as: Anal Biochem. 1998 Nov 1;264(1):87–97. doi: 10.1006/abio.1998.2793

Figure 1.

Figure 1

Nucleotide and amino acid sequences for constructs described in this paper. (A) pMal-pIII vector with pIII leader sequence, including KpnI(Acc65I) and EagI sites. (B) Ph.D. phage display library with random peptide sequence [Xn, (NNK)n; K = G or T] followed by a GGGS linker, which is inserted between the pIII leader sequence and the mature protein. (C) pMal-X and pMal-KHP vectors with MBP:pVIII chimeric leader sequence with pVIII-derived residues in bold. (D) Phage-display vector f88.4 with pVIII leader sequence. (E) pVIII-displayed peptide library [Xn, (NNK)n] in f88.4. Leader peptidase cleavage sites (^) are indicated.